Search Results - (Author, Cooperation:C. J. Grimmelikhuijzen)
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1Latest Papers from Table of Contents or Articles in PressK. M. Kapheim ; H. Pan ; C. Li ; S. L. Salzberg ; D. Puiu ; T. Magoc ; H. M. Robertson ; M. E. Hudson ; A. Venkat ; B. J. Fischman ; A. Hernandez ; M. Yandell ; D. Ence ; C. Holt ; G. D. Yocum ; W. P. Kemp ; J. Bosch ; R. M. Waterhouse ; E. M. Zdobnov ; E. Stolle ; F. B. Kraus ; S. Helbing ; R. F. Moritz ; K. M. Glastad ; B. G. Hunt ; M. A. Goodisman ; F. Hauser ; C. J. Grimmelikhuijzen ; D. G. Pinheiro ; F. M. Nunes ; M. P. Soares ; E. D. Tanaka ; Z. L. Simoes ; K. Hartfelder ; J. D. Evans ; S. M. Barribeau ; R. M. Johnson ; J. H. Massey ; B. R. Southey ; M. Hasselmann ; D. Hamacher ; M. Biewer ; C. F. Kent ; A. Zayed ; C. Blatti, 3rd ; S. Sinha ; J. S. Johnston ; S. J. Hanrahan ; S. D. Kocher ; J. Wang ; G. E. Robinson ; G. Zhang
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-05-16Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Amino-Acid N-Acetyltransferase ; Animals ; Bees/classification/*genetics ; DNA Transposable Elements ; *Evolution, Molecular ; Gene Expression Regulation ; Gene Regulatory Networks ; *Genetic Drift ; Genome, Insect/genetics ; Phylogeny ; Selection, Genetic ; *Social Behavior ; Transcription Factors/chemistry/genetics ; *TranscriptomePublished by: -
2Articles: DFG German National Licensesde Laat, S. W. ; Tertoolen, L. G. J. ; Grimmelikhuijzen, C. J. P.
[s.l.] : Nature Publishing Group
Published 1980Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Electrotonic coupling between epithelial cells was measured by conventional intracellular glass microelectrodes21. Current pulses were passed and voltages recorded through a single microelectrode. In the intact animal measurements were performed in the head, mid-gastric and foot regions with the ...Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1432-041XKeywords: Hydra mutant ; Morphogenetic substances ; Head formationSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary A mutant ofHydra attenuata is analysed, theaberrant, which is distinct from the wild type in having a smaller head with fewer tentacles and only half the number of head-specific cells. The rate of head and foot regeneration and the doubling time are slower inaberrants than in normal hydra. The lower head-forming potential is paralleled by a reduced concentration of head-specific morphogens: compared to the wild type, in theaberrant the concentration of head activator is reduced to 70% in the head and to 50% in the body, the concentration of head inhibitor is reduced to 50% in the head and to 80% in the body. Theaberrant is more sensitive (3 times) to added head activator and less sensitive (〉5 times) to added head inhibitor than the wild type. The slower rate of foot regeneration is paralleled by a lower content of foot-specific morphogens: compared to the wild type, in theaberrant the foot activator is reduced to 40% and the foot inhibitor to 70%.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1432-041XKeywords: Hydra mutant ; Morphogenetic substances ; Bud formationSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary Non-budding mutants ofChlorohydra viridissima regenerate heads 6 h faster thanHydra attenuata and the number of tentacles per head is higher. The polarity in pieces from the gastric region is the more labile, the smaller the pieces are. In regenerates heads and tentacles form much more frequently than feet, giving rise to bipolar or multiheaded structures. Buds very seldom form under normal conditions, but they occasionally occur in regenerating animals with two cut surfaces. The higher head-forming potential in the mutant is paralleled by a higher head-activator concentration (20-fold in head, 4-fold in body), than inHydra attenuata, which is not accompanied by an equivalent increase in head-inhibitor concentration (1.4-fold in head, 2-fold in body). The foot-activator concentration is slightly reduced (1.3-fold), the foot-inhibitor concentration is higher (1.6-fold) than inH. attenuata. The mutant is extremely insensitive to head activator, relatively insensitive to head inhibitor and foot inhibitor, but sensitive to foot activator.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1432-041XKeywords: Hydra mutants ; Morphogenetic substances ; Size regulationSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary Minis have a doubling time of 2.4 days,maxis of 8 days.Minis regenerate head and foot 5 h faster thanmaxis.Minis have fewer tentacles (4.6) per head thanmaxis (6.1). Inmini the concentration of the head activator is equal to that inHydraattenuata, but the concentration of the head inhibitor is reduced (2-fold in the head, 1.2-fold in the body). The concentrations of the foot factors are normal.Minis respond likeHydra attenuata to head activator, foot activator and foot inhibitor, but they are less sensitive to head inhibitor Inmaxi the concentration of the head activator is higher than inmini (5-fold in the head, 10-fold in the body) and that of the head inhibitor is also higher (4-fold in the head, 5-fold in the body). The concentration of the foot inhibitor is normal, that of the foot activator is doubled.Maxis are insensitive to added head activator and less sensitive to added head inhibitor and foot inhibitor. Their response to foot activator is normal.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesGrimmelikhuijzen, C. J. P. ; Balfe, A. ; Emson, P. C. ; Powell, D. ; Sundler, F.
Springer
Published 1981Staff ViewISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Using immunocytochemistry we find substance P-like material in nerve cells of hydra. These nerve cells are situated in the ectoderm of the basal disk and tentacles. Radioimmunoassay of hydra extracts gives dilution curves parallel to that of synthetic substance P, from which it can be calculated that one animal contains at least 0.6 fmol substance P-like immunoreactivity. After chromatography on Biogel P-100, the substance P-like immunoreactivity elutes as a peak in the void volume and a peak at the position of synthetic substance P.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Neurotensin-like immunoreactivity is found in nerve fibers present in all body regions of hydra. The nerve fibers are especially numerous in the ectoderm at the bases of the tentacles and in the ectoderm at a site just above the foot. Radioimmunoassays of acetic-acid extracts of hydra, using various region-specific antisera towards mammalian neurotensin, show the presence of multiple neurotensin-related peptides. The amounts of these peptides vary between 1 and 350 pmol per gram wet weight. Gel filtration on Sephadex G-25 reveals a fraction of neurotensin-like peptides that crossreacts equally well with an antiserum directed against sequence 1–8 and an antiserum directed against sequence 6–13 of neurotensin. This fraction elutes also at the position of neurotensin and might closely resemble the mammalian peptide. A fraction eluting with the void volume crossreacts preferentially with antisera directed against sequences 1–8 and 10–13 of neurotensin. Several components of apparent lower molecular weight than neurotensin crossreact preferentially with an antiserum against sequence 10–13. These last peptides represent the major portion of the neurotensin-like peptides in hydra.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Abundant FMRFamide immunoreactivity has been found in the nervous systems of all hydrozoan, anthozoan, scyphozoan and ctenophoran species that were looked upon. This general and abundant occurrence shows that FMRFamide-like material must play a crucial role in the functioning of primitive nervous systems.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Several reports (cf. Weber et al. (1981) Science 214: 1248–1251) have described the extensive occurrence, in rat brain, of material immunologically related to the molluscan neuropeptide FMRFamide. We have reexamined these data in guinea pig and rat, using six different antisera to FMRFamide. Immunoreactive perikarya and fibres were found to be distributed throughout the rodent brain (Table 1). This distribution was roughly similar to that found by Weber and coworkers. However, solid-phase absorption of the antisera with bovine pancreatic polypeptide, which shares an arginine and an amidated aromatic amino acid (RYamide) with FMRFamide, showed that staining in most regions could be due to crossreactivity with bovine pancreatic polypeptide-like (or neuropeptide Y-like) material. Double-labelling experiments with antisera to FMRFamide and bovine pancreatic polypeptide led to the same conclusion. The only structures where no apparent crossreactivity occurred were perikarya and fibres in the nucleus dorsomedialis, ventromedialis, periventricularis and paraventricularis hypothalami and fibres in the area lateralis hypothalami, nucleus parabrachialis, substantia grisea centralis mesencephali, various parts of the formatio reticularis, and spinal cord. Hence only these structures might contain material which is more related to the molluscan retrapeptide.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary FMRFamide-like immunoreactivity has been localized in different parts of the hydra nervous system. Immunoreactivity occurs in nerve perikarya and processes in the ectoderm of the lower peduncle region near the basal disk, in the ectoderm of the hypostome and in the ectoderm of the tentacles. The immunoreactive nerve perikarya in the lower peduncle region form ganglion-like structures. Radioimmunoassays of extracts of hydra gave displacement curves parallel to standard FMRFamide and values of at least 8 pmol/gram wet weight of FMRFamide-like immunoreactivity. The immunoreactive material eluted from Sephadex G-50 in several components emerging shortly before or after position of authentic FMRFamide. The presence of FMRFamide-like material in coelenterates shows that this family of peptides is of great antiquity.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Using immunocytochemistry, gastrin/CCK-like immunoreactivity is found in sensory nerve cells in the ectoderm of the mouth region of hydra and in nerve cells in the endoderm of all body regions of the sea anemone tealia. These results are corroborated by radioimmunoassay: One hydra contains at least 5 fmole and one tealia at least 2 nmole gastrin/CCK-like immunoreactivity. Reactivities towards gastrin and CCK antisera with different specificities suggest that the coelenterate gastrin/CCK-like peptide contains the C-terminal amino-acid sequence common to mammalian gastrin and CCK. In addition the radioimmunochemical data indicate that the coelenterate peptide also contains an amino-acid sequence that resembles the sequence 20–30 of porcine CCK-33, but that no other sequences of gastrin are present. Thus, it is probably more CCK-like than gastrin-like.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1432-119XSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary With immunocytochemical methods, nerve cells have been detected in Hydra attenuata containing bombesin-like immunoreactivity. These nerve cells are located in the ectoderm of all body regions of the animal and are especially abundant in basal disk and tentacles. Radioimmunoassay of extracts of hydra demonstrated at least 0.2 pmol/g wet weight of bombesinlike immunoreactivity. The immunoreactive material elutes from Sephadex G-50 in a similar position to synthetic bombesin. The data show that bombesin-like peptides are among the phylogenetically oldest neuropeptides found so far.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1432-0878Keywords: RFamide ; Neuropeptide ; Nervous system ; Siphonophores ; CoelenteratesSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary An antiserum to the sequence Arg-Phe-amide (RFamide) was used to stain the nervous systems of various physonectid siphonophores. In the stem of Nanomia bijuga, this antiserum stained an ectodermal nerve net, which was interrupted, at regular intervals, by transverse collars of neurons. Injection of Lucifer yellow into the “giant axon” of the stem showed that this axon was dye-coupled to an ectodermal nerve net that resembled the RFamide-positive network. Ectodermal nets of neurons were also found in the pneumatophore, gastrozooids, tentacles and tentilla. At the junctions of the pneumatophore, the gastrozooids, the dactylozooids and the gonozooids with the stem, and at the junctions of tentacles and tentilla, collars or rings of neurons occurred. The stem was connected to the phyllozooids and nectophores by muscular lamellae, which were bordered by chains of neurons. At the margin of the nectophores, an immunoreactive nerve ring was found. Connected to this ring and located in the“seitliche Zapfen” (“sidely-located patche”), were two agglomerations of nerve cells. On the upper side of the bell margin, positioned at 90° relative to the “seitliche Zapfen”, a delta-shaped neuronal structure was found. This structure was connected to the nerve ring and was associated with a muscle, which ran a short distance along the exumbrellar surface. The nervous systems of Agalma elegans, Forskalia edwardsi, Forskalia leuckarti and Halistemma rubrum resembled that of Nanomia bijuga in all major respects.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesJirikowski, G. ; Erhart, G. ; Grimmelikhuijzen, C. J. P. ; Triepel, J. ; Patzner, R. A.
Springer
Published 1984Staff ViewISSN: 1432-0878Keywords: Hagfish ; Brain ; Pituitary ; FMRF-amide ; ImmunocytochemistrySource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Paraffin sections of brain and pituitary of the hagfish Eptatretus burgeri were immunostained with an antiserum to FMRF-amide. Immunoreactivity was visible in a large number of neurons in the posterior part of the ventromedial hypothalamus and in long neuronal processes extending cranially from the hypothalamus to the olfactory system and caudally to the medulla oblongata. FMRF-amide-like immunoreactivity was also found in cells of the adenohypophysis. These observations suggest that the hagfish possesses a brain FMRF-amide-like transmitter system and pituitary cells containing FMRF-amide-like material. Antisera to ACTH, α-MSH and pancreatic polypeptide gave no immunoreaction in hagfish brain or pituitary.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1432-0878Keywords: FMRFamide ; Pancreatic polypeptide ; Hypothalamus ; Neuropeptide immunocytochemistry ; Brain mapping ; Guinea pigSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Immunoreactive neurons were mapped in the central nervous system of colchicine-treated and untreated guinea pigs with the use of two antisera to the molluscan neuropeptide FMRFamide 1. These antisera were especially selected for their incapability to react with peptides of the pancreatic polypeptide family. Only one group of perikarya was stained by both antisera; this group was mainly located in the nucleus dorsomedialis hypothalami and extended to the nucleus paraventricularis and nucleus periventricularis hypothalami. The perikarya were found to project fibers to all regions of the hypothalamus, to the septum, nucleus proprius striae terminalis, nucleus paraventricularis thalami, nucleus centralis thalami, nucleus reuniens, medial, central and basal amygdala, area praetectalis, area tegmentalis ventralis of Tsai, substantia grisea centralis mesencephali, formatio reticularis mesencephali, nucleus centralis superior, locus coeruleus, nuclei parabrachiales, nucleus raphe magnus, A 5-region, vagus-solitarius complex, ventral medulla, nucleus spinalis nervi trigemini, and substantia gelatinosa of the spinal cord. In many brain regions FMRFamide-immunoreactive processes were found in close contact with blood vessels.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1432-0878Keywords: RFamide ; Neuropeptides ; Nervous system ; Reproduction ; HydrozoaSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Antisera to the sequence Arg-Phe-amide (RF-amide) have a high affinity to the nervous system of fixed hydroid polyps. Whole-mount incubations of several Hydra species with RFamide antisera visualize the three-dimensional structure of an ectodermal nervous system in the hypostome, tentacles, gastric region and peduncle. In the hypostome of Hydra attenuata a ganglion-like structure occurs, consisting of numerous sensory cells located in a region around the mouth opening and a dense plexus of processes which project mostly radially towards the bases of the tentacles. In Hydra oligactis an ectodermal nerve ring was observed lying at the border of hypostome and tentacle bases. This nerve ring consists of a few large ganglion cells with thick processes forming a circle around the hypostome. This is the first direct demonstration of a nerve ring in a hydroid polyp. Incubation of Hydractinia echinata gastrozooids with RFamide antisera visualizes an extremly dense plexus of neuronal processes in body and head regions. A ring of sensory cells around the mouth opening is the first group of neurons to show RFamide immunoreactivity during the development of a primary polyp. In gonozooids the oocytes and spermatophores are covered with strongly immunoreactive neurons. All examples of whole-mount incubations with RF-amide antisera clearly show that hydroid polyps have by no means a “diffuse nerve net”, as is often believed, and that neuronal centralization and plexus formation are common in these animals. The examples also show that treatment of intact fixed animals with RFamide antisera is a useful technique to study the anatomy or development of a principal portion of the hydroid nervous system.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesGrimmelikhuijzen, C. J. P. ; Graff, D. ; Koizumi, O. ; Westfall, J. A. ; McFarlane, I. D.
Springer
Published 1991Staff ViewISSN: 1573-5117Keywords: Neuropeptides ; neurones ; coelenteratesSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Coelenterate neurones produce peptides containing an Arg-Phe-NH2(RF-amide)-like carboxyterminus. RF-amide-like peptides are located in neuronal dense-cored vesicles, indicating that they are released by exocytosis and that they might function as neurotransmitters or neurohormones. Using a radioimmunoassay for the sequence RF-amide, 3 peptides were isolated from the sea anemone Anthopleura elegantissima: 〈 Glu-Gly-Arg-Phe-NH2(Antho-RF-amide), 〈Glu-Ser-Leu-Arg-Trp-NH2(Antho-RWamide I) and 〈Glu-Gly-Leu-Arg-Trp-NH2(Antho-RW-amide II). The general structure of these peptides can be described as 〈Glu...Arg-X-NH2, where X is an aromatic amino acid. From the hydromedusa Polyorchis penicillatus, the peptide 〈Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2(Pol-RF-amide I) was isolated, which also belongs to the 〈Glu...Arg-X-NH2 family. Using specific antisera, it was shown that all 4 peptides were located in neurones, many of which were associated with smooth muscle fibres. Application of low doses of Antho-RF-amide or of Antho-RW-amide I and II induced contractions of endodermal muscles of sea anemones. This suggests that these peptides are transmitters or modulators at neuromuscular junctions.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1432-0878Keywords: Cell junctions ; Epithelial cell ; Nerve-free hydraSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Epithelial cells of nerve-free hydra contain septate and gap junctions. In thin sections the gap junctions are characterized by a gap of 3–4 nm. Freeze-fracture demonstrates the presence of septate junctions and two further types of structures: (i) the “E-type” or “inverted” gap junctions with particles in an “en plaque” conformation appearing as a raised plateau on the E-face or as a depression on the P-face; (ii) structures morphologically similar to gap junctions in rat liver, containing particles on the P-face and corresponding pits on the E-face, both having hexagonal packing with a lattice constant of 8 nm. We propose that these structures are also gap junctions.Type of Medium: Electronic ResourceURL: