Search Results - (Author, Cooperation:C. F. Harvey)

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  1. 1
    Staff View
    Publication Date:
    2013-09-13
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Arsenic/*analysis ; Arsenic Poisoning ; Carbon/analysis ; Drinking Water/chemistry ; Food Contamination/analysis ; Geologic Sediments/chemistry ; Groundwater/analysis/*chemistry ; Humans ; Oxidation-Reduction ; Rivers/chemistry ; Silicon Dioxide/analysis/chemistry ; Vietnam ; Water Movements ; Water Wells/chemistry
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    ISSN:
    1432-2145
    Keywords:
    Key words Actinidia ; Dioecism ; Sex determination ; Sex markers ; Sex ratios
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  First results from two strategies aimed at elucidating the genetics of sex in the dioecious genus Actinidia Lindl. (Actinidiaceae) support the hypothesis that sex-determining genes are localized in a pair of chromosomes which, although cytologically indistinguishable, function like an XX/XY system with male heterogamety. A. chinensis Planch., a close relative of the kiwifruit [A. deliciosa (A. Chev.) CF Liang et AR Ferguson], has diploid and tetraploid races. Bulk segregant analysis to find sex-linked markers revealed two markers whose inheritance patterns in three diploid families showed X and Y linkage and indicated that the male is the heterogametic sex. Some recombination between the markers and the sex-determining loci was also demonstrated. Sex ratios in 12 progenies from controlled crosses varied around 1:1, as expected for an XX/XY system.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s004970050082

    Articles: DFG German National Licenses
  3. 3
    Gill, G. P. ; Harvey, C. F. ; Gardner, R. C. ; Fraser, L. G.
    Springer
    Published 1998
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Actinidia ; RAPD ; SCAR ; Sex-linked markers ; Marker-assisted selection
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  Two sex-linked random amplified polymorphic DNA (RAPD) markers identified from Actinidia chinensis were converted into sequence-characterised amplified regions (SCARs) for the large-scale screening of Actinidia breeding populations. Initial SCAR primers converted one RAPD (SmX) into a dominant marker, but the other (SmY), which was potentially more useful because of its linkage to the male determining ‘Y’ locus, failed to retain polymorphism. This difficulty was overcome by cloning and sequencing the alternate ‘allele’ from female plants, and then designing ‘allele’-specific primers that utilised nucleotide differences between the sexes. Using a quick squash-blot method of DNA extraction, the SCAR primers were tested in 120 A. chinensis plants to determine their gender. The system is now in use for large-scale screening of seedling populations in the Actinidia breeding programme. The sex-linked SCAR primers also functioned with plants from some other geographically separate accessions of A. chinensis and with plants in the closely related polyploid species A. deliciosa, but did not amplify a sex-linked band in more distantly related species of Actinidia.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050914

    Articles: DFG German National Licenses