Search Results - (Author, Cooperation:B. Vandenbussche)

2012-10-06

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Electronic Resource

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

A genetic linkage map based on an intraspecific cross between two inbred lines of witloof-chicory (Cichorium intybus L. var. foliosum Hegi) has been constructed. In total, 129 RAPD markers were scored in 565 F2 plants. Grouping of these markers at a LOD of threshold 4.0 resulted in nine linkage groups, which is equal to the chicory haploid genome. The nine linkage groups covered 609.6 cM. All 129 RAPD markers were linked to one of the nine groups. Three RAPD markers could not be mapped. Out of the 126 remaining RAPD markers, 18 showed segregation distortion with significance value of P 〈 0.01.

Electronic Resource

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

The genetic basis of pith characteristics in chicory (Cichorium intybus L. var. foliosum Hegi) was investigated. Quantitative trait loci (QTL) were mapped in an F2 population (565 F2 plants) derived from a cross between two inbred chicory lines. A molecular marker linkage map of this cross had previously been constructed based on 129 random amplified polymorphic DNA markers. Each F2 plant was selfed and plant characteristics were measured in the F3 populations. Although variation in pith characteristics was largely environmentally influenced, QTL for the characteristics length of pith, browning of the pith, hollow pith and apple pith were detected in many linkage groups. Interactions between QTL were found for the three characteristics: pith length, browning of the pith and hollow pith. The QTL detected confirmed the early forcing suitability of the one parent inbred line and late forcing suitability of the other.

Electronic Resource

1572-946X

Springer Online Journal Archives 1860-2000

Physics

Electronic Resource

1432-203X

Key wordsBeta vulgaris L. ; Cryopreservation ; Encapsulation-dehydration technique ; Abscisic acid ; Cold acclimation

Springer Online Journal Archives 1860-2000

Biology

Abstract It has been previously shown that shoot tips of in vitro plantlets of sugar beet (Beta vulgaris L. clone SES1) can be cryopreserved using the encapsulation-dehydration technique (survival rate of 37% after freezing). This article reports the influence of abscisic acid (ABA) and cold acclimation on survival after cryopreservation. When ABA was added to the multiplication medium of the plants, the survival rate of shoot tips after cryopreservation was not increased (45%). After cold acclimation of the plants, their growth pattern differed (plants became apically dominant) and the survival rate of the shoot tips after cryopreservation clearly increased (70% survival and 50% plant regeneration after freezing). This improved protocol was successfully applied to three other clones.

Electronic Resource

1432-203X

Key words Beta vulgaris L. Cryopreservation ; Shoot tips ; Encapsulation-dehydration ; Vitrification ; AbbreviationsBA: N-6-benzylaminopurine ; CMS:¶Cytoplasmic male sterile ; DMSO: Dimethylsulfoxide ; DGJ: Medium of De Greef and Jacobs (1979) ; DGJ0: Medium of De Greef and Jacobs (1979) lacking BA ; IBA: Indole-3-butyric acid ; kin: 6-Furfurylaminopurine ; LS: Loading solution ; PAR: Photosynthetic active radiation ; PVS2: Plant vitrification solution ; RS: Recovery solution

Springer Online Journal Archives 1860-2000

Biology

Abstract  Sugar beet shoot tips from cold-acclimated plants were successfully cryopreserved using a vitrification technique. Dissected shoot tips were precultured for 1 day at 5  °C on solidified DGJ0 medium with 0.3 M sucrose. After loading for 20 min with a mixture of 2 M glycerol and 0.4 M sucrose (20  °C), shoot tips were dehydrated with PVS2 (0  °C) for 20 min prior to immersion in liquid nitrogen. Both cold acclimation and loading enhanced the dehydration tolerance of shoot tips to PVS2. After thawing, shoot tips were deloaded for 15 min in liquid DGJ0 medium with 1.2 M sucrose (20  °C). The optimal exposure time to both loading solution and PVS2 depended on the in vitro morphology of the clone. With tetraploid clones a higher sucrose concentration during cold acclimation and preculture further enhanced survival after cryopreservation. Survival rates ranged between 60% and 100% depending on the clone. Since only 10–50% of the surviving shoot tips developed into non-hyperhydric shoots, regrowth was optimized.

Electronic Resource

1573-5087

Beta vulgaris L. ; cryopreservation ; cold acclimation ; fatty acids ; sugars

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Abstract To cryopreserve sugar beet shoot tips using an encapsulation-dehydration technique, cold hardening of in vitro plants was needed to obtain high survival rates after freezing. Cold acclimation not only enhanced dehydration and freezing tolerance, but also induced several changes in sugar beet shoots. Plants contained greater amounts of sucrose, D-glucose and D-fructose and the fatty acid composition of lipids changed. Furthermore, the unsaturation level of membrane lipids, estimated by the (C18:2 + C18:1)/C16:0 ratio, increased after cold hardening. These changes were correlated with better survival rates after cryopreservation.

Electronic Resource

1572-9508

infrared detectors ; radiation ; space environment

Springer Online Journal Archives 1860-2000

Physics

Abstract We present a trend analysis of the ISO-SWS detector performance and a study of the space radiation effects on the SWS detectors. In particular, dark currents, dark current noise and detector responses have been checked as a function of time through the mission and as a function of time in arevolution. The results show that these parameters were stable during the mission in all bandsbut for band 3 (Si:As). Dark currents and responses were found to be higherin the first hours following the start of the science window,especially in band 2 (Si:Ga). We have studied the impacts of cosmic rays and radiation belt particles on the SWS detectors, as well as of the only large solar proton event on November 6, 1997,that occurred during the ISO mission (operated during solar minimum).The observed glitch rates in all SWS bands are found to be between 2 and4 times higher than the value predicted by the CREME96 model for the cosmic ray flux in the period considered. The bands that registered the highest glitch rates showed also a correlation with the electron fluxes measured on theGOES 9 spacecraft. From the distribution of glitchheights (voltage jumps in the detector signal), we have derived the deposited energy distributions of the particles hits. Our results lead to the conclusion that secondaryparticles produced in the shield and the detectors contributed at least as much as cosmic rays to the observed glitch rate. The effects on the detectors of the November 6, 1997 event, which caused that all observationsin a revolution were declared failed, are described in detail.

Electronic Resource