Search Results - (Author, Cooperation:B. Hamprecht)

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  1. 1
    Staff View
    Publication Date:
    2012-05-25
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Action Potentials ; Alkyl and Aryl Transferases/deficiency/genetics/metabolism ; Animals ; Axons/*physiology ; Brain/cytology/metabolism ; Cell Respiration ; Cell Survival ; Demyelinating Diseases/enzymology/genetics/metabolism/pathology ; Electron Transport Complex IV/antagonists & inhibitors/genetics/metabolism ; *Glycolysis ; Lactic Acid/metabolism ; Magnetic Resonance Spectroscopy ; Membrane Proteins/deficiency/genetics/metabolism ; Mice ; Mitochondria/enzymology/genetics/metabolism/pathology ; Mutant Proteins/genetics/metabolism ; Myelin Sheath/*metabolism ; Oligodendroglia/cytology/drug effects/enzymology/*metabolism ; Protons ; Schwann Cells/enzymology/metabolism ; Time Factors
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Vogel, R. ; Jennemann, G. ; Seitz, J. ; Wiesinger, H. ; Hamprecht, B.

    Oxford, UK : Blackwell Science Ltd
    Published 1998
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract: To elucidate the cellular location of mitochondrial malic enzyme in brain, immunocytochemical studies were performed. For this purpose, mitochondrial malic enzyme was purified to apparent homogeneity from bovine brain and used for the immunization of rabbits. Subjecting the antiserum to affinity purification on immobilized antigen as an absorbent yielded a purified immunoreactive antibody preparation, which was characterized by probing cytosolic and mitochondrial fractions of bovine and rat brain in western blotting. As neither crossreactivity with cytosolic malic enzyme nor immunoreactivity against other proteins could be observed, the antibody preparation was found suitable for immunocytochemistry. By using sections of perfusion-fixed rat brain, considerable resolution was achieved at the light-microscopic level. Distinct and specific staining of neurons was observed; in contrast, no staining of astrocytes and possibly unspecific staining within the nuclei of oligodendrocytes were obtained. From these data, it is concluded that mitochondrial malic enzyme is located in neurons; however, in astrocytes, the enzyme appears to be either lacking or present at a much lower level. A protective role against oxidative stress in neurons is proposed for mitochondrial malic enzyme.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1471-4159.1998.71020844.x

    Articles: DFG German National Licenses
  3. 3
    Reiser, G. ; Scholz, F. ; Hamprecht, B.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1982
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract: Interaction of Li+ with the voltage-dependent Na+ channel has been analyzed in neuroblastoma × glioma hybrid cells. The cells were able to generate action potentials in media containing Li+ instead of Na+. The uptake of Li+ into the hybrid cells was investigated for the pharmacological analysis of Li+ permeation through voltage-dependent Na+ channels. Veratridine and acionitine increased the uptake of Li+ to the same degree (EC50 30 μM). This increase was blocked by tetrodotoxin (IC50 20 nM). Veratridine and aconitine did not act synergistically; however, the veratridine-stimulated influx was further enhanced by the toxin of the scorpion Leiurus quinquestriatus (EC50 0.06 μg/ml). This stimulation was also blocked by tetrodotoxin. Thus, the voltage-dependent Na+ channel of the hybrid cells accepts both Li+ and Na+ in a similar manner.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1982.tb04723.x

    Articles: DFG German National Licenses
  4. 4
    Calker, D. ; Müller, M. ; Hamprecht, B.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1978
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract— The β-adrenergic agonist, isoproterenol and the α- and β-adrenergic agonist. NA. raise the intracellular concentration of cyclic AMP in cultures of dissociated perinatal mouse brain. This rise is prevented by a β- but not by an α-adrenergic antagonist. The maximal level of cyclic AMP reached in the presence of isoproterenol is markedly higher than that found after exposure to NA. However, if NA is used along with an α-adrenergic antagonist, cyclic AMP levels as high as those after isoproterenol are measured. Agonists with α-adrenergic activity including NA decrease the response to isoproterenol. The decrease is blocked by α-adrenergic antagonists. From this and additional evidence it is concluded: (1) The increase in the level of cyclic AMP caused by β-adrenergic agonists is due to β-receptor-mediated stimulation of adenylate cyclase; (2) the inhibition of this effect by α-adrenergic agonists is mediated by adrenergic α-receptors; (3) the α- and β-adrenergic receptors are likely to be located on the same cells, probably the most abundant putative glial precursor cells. The simultaneous stimulation of α- and β-adrenergic receptors on the same cell may be of significance in the regulation of the response to NA.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1978.tb10776.x

    Articles: DFG German National Licenses
  5. 5
    Hamprecht, B.

    Oxford, UK : Blackwell Publishing Ltd.
    Published 2005
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1474-1644.2005.03229_6.x

    Articles: DFG German National Licenses
  6. 6
    Brandt, M. ; Buchen, C. ; Hamprecht, B.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1980
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract: Neuroblastoma × glioma hybrid cells increase their intracellular concentration of cyclic AMP in response to prostaglandin E1 (PGE1). This effect is inhibited by opioids. The response to PGE1 is positively correlated with the concentration of Ca2+ in the incubation medium. The Ca2+ antagonists Co2+ and La3+, the Ca2+ chelator EGTA and a blocker of Ca2+ influx into cells, Segontin, inhibit the response to PGE1. At low external concentrations of Ca2+ the response to PGE1 is enhanced by the Ca2+ ionophore A23187. The effects of A23187 and Segontin point to a cytosolic site of Ca2+ action. Lack of Ca2+ reduces the level of cyclic AMP even in the absence of PGE1 and the presence of an inhibitor of cyclic AMP phosphodiesterase. Ca2+ is required even for an increase in the level of cyclic AMP in cells pretreated with cholera toxin. The increases in level of cyclic AMP evoked by PGE, in a neuroblastoma and by PGE1 or noradrenaline in a glioma cell line do not depend on Ca2+. The response of the hybrid cells to the opioid leucine-enkephalin appears not to rely on the presence of Ca2+. Even changing the intracellular concentration of Ca2+ by the ionophore A23187 does not alter the effect of the opioid. The analogy between opioids and lack of Ca2+ in the short-term (minutes) experiments mentioned holds also for long-term (hours) experiments. Cells chronically exposed to opioids or to low concentrations of Ca2+ display an enhanced maximal response to PGE1.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1980.tb11192.x

    Articles: DFG German National Licenses
  7. 7
    Horsthemke, B. ; Hamprecht, B. ; Bauer, K.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0006-291X
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/S0006-291X(83)80161-2

    Articles: DFG German National Licenses
  8. 8
    Broer, S. ; Broer, A. ; Hamprecht, B.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0005-2736
    Keywords:
    Amino acid transporter ; Astroglial cell ; C6-BU-1 cell ; Expression ; Oocyte
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0005-2736(94)90147-3

    Articles: DFG German National Licenses
  9. 9
    HAMPRECHT, B.

    [s.l.] : Nature Publishing Group
    Published 1977
    Staff View
    ISSN:
    1476-4687
    Source:
    Nature Archives 1869 - 2009
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Notes:
    [Auszug] IN September 1976 Dr Robert J. Gullis left our laboratory after having spent two postdoctoral years with us. He had been engaged mainly in measuring the levels of cyclic GMP in neuroblastoma cells and neuroblastoma X glioma hybrid cells. We published four papers on this matter together with him. ...
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1038/265764b0

    Articles: DFG German National Licenses
  10. 10
    Hamprecht, B. ; Amano, T.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0003-2697
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0003-2697(74)90062-1

    Articles: DFG German National Licenses
  11. 11
    Hamprecht, B. ; Schultz, J.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(73)80709-4

    Articles: DFG German National Licenses
  12. 12
    Reiser, G. ; Hamprecht, B.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(82)90243-9

    Articles: DFG German National Licenses
  13. 13
    Reiser, G. ; Gunther, A. ; Hamprecht, B.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-5793
    Keywords:
    Benzocaine ; Dibucaine ; Lidocaine ; Tetracaine ; Voltage-dependent sodium channel
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(82)80122-1

    Articles: DFG German National Licenses
  14. 14
    Hamprecht, B. ; Jaffe, B.M. ; Philpott, G.W.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(73)80367-9

    Articles: DFG German National Licenses
  15. 15
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(77)80186-5

    Articles: DFG German National Licenses
  16. 16
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(77)80552-8

    Articles: DFG German National Licenses
  17. 17
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(76)80399-7

    Articles: DFG German National Licenses
  18. 18
    Heumann, R. ; Reiser, G. ; Van Calker, D. ; Hamprecht, B.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(82)90325-1

    Articles: DFG German National Licenses
  19. 19
    Hamprecht, B. ; Nussler, C. ; Lynen, F.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-5793
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(69)80210-3

    Articles: DFG German National Licenses
  20. 20
    Amano, T. ; Hamprecht, B. ; Kemper, W.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0014-4827
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0014-4827(74)90142-6

    Articles: DFG German National Licenses