Search Results - (Author, Cooperation:B. Gonzalez)

2015-07-23

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

3' Untranslated Regions/genetics ; Alternative Splicing/genetics ; B-Cell-Specific Activator Protein/biosynthesis/genetics ; B-Lymphocytes/metabolism ; Carrier Proteins/genetics ; Chromosomes, Human, Pair 9/genetics ; DNA Mutational Analysis ; DNA, Neoplasm/genetics ; Enhancer Elements, Genetic/genetics ; Genomics ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/metabolism/pathology ; Mutation/*genetics ; Nerve Tissue Proteins/genetics ; Nuclear Proteins/genetics ; Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics ; Receptor, Notch1/genetics/metabolism ; Transcription Factors/genetics

2014-11-08

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Anti-Bacterial Agents/pharmacology ; B-Lymphocytes/immunology/*virology ; Caliciviridae Infections/*immunology/microbiology/virology ; Cell Line ; Enterobacteriaceae/drug effects/*physiology ; Gastroenteritis/*immunology/microbiology/virology ; Genome, Viral/genetics/physiology ; Homeodomain Proteins/genetics ; Humans ; Intestines/immunology/*microbiology ; Mice ; Mice, Mutant Strains ; Norovirus/*physiology ; Peyer's Patches/immunology/virology ; *Virus Replication

1365-2494

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Changes in stubble carbohydrate content during the regrowth of ryegrass (Lolium perenne L.) grown under hydroponic conditions at two nitrogen levels were studied as a function of time by high-performance liquid chromatography. Experimental data showed that regrowth at a non-limiting nitrogen level (1·0 mol m−3 NH4NO3) involved two different physiological periods. The first occurred during the first 6 d and was characterized by the mobilization of 60 to 90% of the soluble carbohydrates (i.e. glucose, fructose, sucrose, oligofructans and polyfructans). During the second period (6.28 d of regrowth) carbohydrate contents rose to the values observed prior to cutting (20% of dry matter at the 28th d of regrowth).The effect of low nitrogen conditions (0·2 mol m−3 NH4NO3) was observed only during the second phase. Plants regrown in a nitrogen-starved medium accumulated 2·3-fold more polyfructans than plants regrown in a non-limiting nitrogen medium. Their fructose and glucose contents remained at 2% of dry matter from the end of the first phase of mobilization.The experimental results are interpreted and discussed in terms of the existence of two distinct fructan synthetic pathways.

Electronic Resource

1749-6632

Blackwell Publishing Journal Backfiles 1879-2005

Natural Sciences in General

Electronic Resource

1460-9568

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Oxidative stress, resulting from accumulation of reactive oxygen species, plays a critical role in neuronal cell death associated with neurodegenerative diseases and stroke. In the present study, we have investigated the potential neuroprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on oxidative stress-induced apoptosis. Incubation of cerebellar granule cells with PACAP inhibited hydrogen peroxide-evoked cell death in a concentration-dependent manner. The effect of PACAP on granule cell survival was not mimicked by vasoactive intestinal polypeptide and was blocked by the antagonist PACAP6-38. The protective action of PACAP upon hydrogen peroxide-induced neuronal cell death was abolished by the MAP-kinase kinase (MEK) inhibitor U0126 and mimicked by the caspase-3 inhibitor Z-DEVD-FMK. PACAP markedly inhibited hydrogen peroxide-evoked caspase-3 activation and DNA fragmentation. Taken together, these data indicate that PACAP, acting through PACAP receptor type 1, exerts a potent protective effect against neuronal degeneration induced by hydrogen peroxide. The anti-apoptotic effect of PACAP is mediated through the MAP-kinase pathway and can be accounted for by inhibition of caspase-3 activation resulting from oxidative stress.

Electronic Resource

1460-9568

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Activation of potassium (K+) currents plays a critical role in the control of programmed cell death. Because pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to inhibit the apoptotic cascade in the cerebellar cortex during development, we have investigated the effect of PACAP on K+ currents in cultured cerebellar granule cells using the patch-clamp technique in the whole-cell configuration. Two types of outward K+ currents, a transient K+ current (IA) and a delayed rectifier K+ current (IK) were characterized using two different voltage protocols and specific inhibitors of K+ channels. Application of PACAP induced a reversible reduction of the IK amplitude, but did not affect IA, while the PACAP-related peptide vasoactive intestinal polypeptide had no effect on either types of K+ currents. Repeated applications of PACAP induced gradual attenuation of the electrophysiological response. In the presence of guanosine 5′-[γthio]triphosphate (GTPγS), PACAP provoked a marked and irreversible IK depression, whereas cell dialysis with guanosine 5′-[βthio]diphosphate GDPβS totally abolished the effect of PACAP. Pre-treatment of the cells with pertussis toxin did not modify the effect of PACAP on IK. In contrast, cholera toxin suppressed the PACAP-induced inhibition of IK. Exposure of granule cells to dibutyryl cyclic adenosine monophosphate (dbcAMP) mimicked the inhibitory effect of PACAP on IK. Addition of the specific protein kinase A inhibitor H89 in the patch pipette solution prevented the reduction of IK induced by both PACAP and dbcAMP. PACAP provoked a sustained increase of the resting membrane potential in cerebellar granule cells cultured either in high or low KCl-containing medium, and this long-term depolarizing effect of PACAP was mimicked by the IK specific blocker tetraethylammonium chloride (TEA). In addition, pre-incubation of granule cells with TEA suppressed the effect of PACAP on resting membrane potential. TEA mimicked the neuroprotective effect of PACAP against ethanol-induced apoptotic cell death, and the increase of caspase-3 activity observed after exposure of granule cells to ethanol was also significantly inhibited by TEA. Taken together, the present results demonstrate that, in rat cerebellar granule cells, PACAP reduces the delayed outward rectifier K+ current by activating a type 1 PACAP (PAC1) receptor coupled to the adenylyl cyclase/protein kinase A pathway through a cholera toxin-sensitive Gs protein. Our data also show that PACAP and TEA induce long-term depolarization of the resting membrane potential, promote cell survival and inhibit caspase-3 activity, suggesting that PACAP-evoked inhibition of IK contributes to the anti-apoptotic effect of the peptide on cerebellar granule cells.

Electronic Resource

1460-9568

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

In rats, rapid eye movement (REM) sleep can be elicited by microinjection of vasoactive intestinal polypeptide (VIP) into the oral pontine reticular nucleus (PnO). In the present study, we investigated whether this area could also be a REM-promoting target for a peptide closely related to VIP: the pituitary adenylyl cyclase-activating polypeptide (PACAP). When administered into the posterior part of the PnO, but not in nearby areas, of freely moving chronically implanted rats, PACAP-27 and PACAP-38 (0.3 and 3 pmol) induced a marked enhancement (60–85% over baseline) of REM sleep for 8 h that could be prevented by prior infusion of the antagonist PACAP-(6–27) (3 pmol) into the same site. Moreover, injections of PACAP into the centre of the posterior PnO resulted in REM sleep enhancement which could last for up to 11 consecutive days. Quantitative autoradiography using [125I]PACAP-27 revealed the presence in the PnO of specific binding sites with high affinity for PACAP-27 and PACAP-38 (IC50 = 2.4 and 3.2 nm, respectively), but very low affinity for VIP (IC50 〉 1 μm). These data suggest that PACAP within the PnO may play a key role in REM sleep regulation, and provide evidence for long-term (several days) mechanisms involved in such a control. PAC1 receptors which have a much higher affinity for PACAP than for VIP might mediate this long-term action of PACAP on REM sleep.

Electronic Resource

1749-6632

Blackwell Publishing Journal Backfiles 1879-2005

Natural Sciences in General

Electronic Resource

1365-4632

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

A 34-year-old-man diagnosed of chronic myeloid leukemia Ph positive in chronic phase achieved hematological complete response after 19 months of hydroxyurea treatment (1.5 g/day). The patient was then admitted to the hospital for an allogenic bone marrow transplantation from an identic Human Leucocyte Antigen (HLA) donor. Conditioning was achieved with busulfan (1 mg/kg/6 h) and cyclophosphamide (60 mg/kg/day). Cyclosporine and methotrexate on short course (days +1, +6, +11) were used to prevent graft-vs.-host disease and acylovir was given as prophylaxis of cytomegalovirus infection. On day +22 he developed bilateral, pruritic, erythema of the palms and erythematous lesions on dorsa of finger joints (〈link href="#f1"〉Fig. 1) and elbows. No periungual erythema was present. There was no evidence of muscle weakness. Lesions disappeared spontaneously in eight days. Laboratory findings were within normal range. Two histological studies taken from palm and elbow showed hyperkeratosis, focal degeneration of the basal cell layer with scattered necrotic keratinocytes and perivascular chronic infiltrate.〈figure xml:id="f1"〉1〈mediaResource alt="image" href="urn:x-wiley:00119059:IJD1349:IJD_1394_f1"/〉Erythema over the interphalangeal joints (case 1)〈section xml:id="abs1-2"〉〈title type="main"〉Case 2A 44-year-old woman on partial remission after chemotherapeutic treatment of a follicular lymphoma stage IVb was admitted to the hospital to undergo peripheral stem cell transplant. Myeloablation and immunosuppression were attained with total body irradiation and cyclophosphamide (4080 mg/24 h). On day +5 after infusion she complained of nonpruritic erythema over eyelids and palpebral edema. A few days later she developed peribucal erythema, erythematous plaques on interdigital folds and painful palmar erythema. Physical examination revealed scaly symmetric erythema over eyelids and erythematous, well defined plaques on interdigital folds, over the interphalangeal joints and elbows (〈link href="#f2"〉Fig. 2). Periungual and palmar erythema was also present. No muscle weakness was evidenced and laboratory studies including muscle enzymes, antinuclear antibodies, Jo-1, Mi-2 and SRP were normal or negative. Skin biopsies from right elbow and right palm showed similar features: hyperkeratosis, vacuolar degeneration of the basal cell layer and chronic perivascular infiltrate (〈link href="#f3"〉Fig. 3).〈figure xml:id="f2"〉2〈mediaResource alt="image" href="urn:x-wiley:00119059:IJD1349:IJD_1394_f2"/〉Erythematous plaque on the elbow (case 2)〈figure xml:id="f3"〉3〈mediaResource alt="image" href="urn:x-wiley:00119059:IJD1349:IJD_1394_f3"/〉Vacuolar degeneration of the basal cell layer and perivascular infiltrates〈section xml:id="abs1-3"〉〈title type="main"〉Case 3A 27-year-old woman diagnosed of acute lymphoblastic leukemia (M4) was admitted to the hospital to undergo chemotherapeutical treatment of a second relapse after peripheral stem cell transplant two years before. High doses of etoposide (5460 mg) were administered for 4 days. The last day of the chemotherapeutic treatment she referred non pruritic erythema over eyelids, cheeks and forehead. On physical examination we could appreciate palpebral edema and symmetric, macular, violaceous, erythema over cheeks, eyelids and forehead (〈link href="#f4"〉Fig. 4). Erythematous, painful plaques on elbows and forearms were also present. No nailfold abnormalities swere observed. Lesions disappeared spontaneously in a few days leaving slight hyperpigmentation. She had no muscular weakness and laboratory findings including muscle enzymes were within normal ranges. Histological studies from left elbow were consistent with a vacuolar interface dermatitis.〈figure xml:id="f4"〉4〈mediaResource alt="image" href="urn:x-wiley:00119059:IJD1349:IJD_1394_f4"/〉Erythema over the eyelids and palpebral edema (case 3)

Electronic Resource

0005-2760

(Rat fetal hepatocyte) ; Albumin ; Hepatoma ; Polyunsaturated fatty acid ; α-Fetoprotein

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0005-2760

(Human T-lymphocyte) ; Blastic transformation ; Fatty acid ; Membrane fluidity

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0005-2760

(Human T-lymphocyte) ; Blastic transformation ; Fatty acid desaturase ; Polyunsaturated fatty acid

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0167-4781

(Erwinia) ; (Synechocystis PCC6803) ; Carotenoid ; Phytoene synthase

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0044-8486

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Electronic Resource

0168-1656

Bacteria involved in lignin degradation ; Degradation of byproducts ; Lignin mineralization ; Novel bacterial enzymes

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Process Engineering, Biotechnology, Nutrition Technology

Electronic Resource

0305-0491

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Electronic Resource

0305-0491

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Electronic Resource

0040-6031

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource