Search Results - (Author, Cooperation:B. Fernandez)

2018-07-19

American Physical Society (APS)

0556-2813

1089-490X

Physics

Nuclear Structure

2018-05-22

American Physical Society (APS)

0556-2813

1089-490X

Physics

Nuclear Reactions

2014-12-06

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; CD4-Positive T-Lymphocytes/*immunology ; Candida albicans/*immunology ; Cells, Cultured ; Clone Cells ; High-Throughput Nucleotide Sequencing ; Host-Pathogen Interactions/*immunology ; Humans ; *Immunologic Memory ; Lymphocyte Activation ; Molecular Sequence Data ; Mycobacterium tuberculosis/*immunology ; Receptors, Antigen, T-Cell/genetics ; T-Lymphocyte Subsets/*immunology ; Th1 Cells/immunology ; Th17 Cells/immunology ; Th2 Cells/immunology ; Vaccines/*immunology

2016-02-27

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Adult ; Animals ; Antibodies, Monoclonal/*administration & dosage/immunology/isolation & ; purification ; Antibodies, Neutralizing/*administration & dosage/immunology/isolation & ; Antibodies, Viral/*administration & dosage/immunology/isolation & purification ; Clinical Trials as Topic ; Disease Outbreaks ; Ebolavirus/*immunology ; Female ; Hemorrhagic Fever, Ebola/epidemiology/*prevention & control ; Humans ; Macaca ; Male ; Molecular Sequence Data ; Survivors

2014-10-09

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adult ; Amino Acid Sequence ; Antibodies, Neutralizing/*genetics ; Cells, Cultured ; Complementarity Determining Regions/chemistry/*genetics ; Female ; Hemagglutinin Glycoproteins, Influenza Virus/immunology ; Humans ; Immunoglobulin Heavy Chains/genetics ; Influenza, Human/*immunology/virology ; Male ; Middle Aged ; Models, Molecular ; Mutation/*genetics ; Orthomyxoviridae/*immunology/metabolism ; Polymorphism, Genetic ; Protein Binding/genetics ; Protein Structure, Tertiary ; Young Adult

2015-12-25

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Antibodies, Monoclonal/chemistry/genetics/*immunology/therapeutic use ; *Antibody Specificity ; Antigenic Variation/*immunology ; Antigens, Protozoan/*immunology ; B-Lymphocytes/cytology/immunology ; Clone Cells/cytology/immunology ; Collagen/immunology/metabolism ; Conserved Sequence/immunology ; DNA Transposable Elements/genetics/immunology ; Epitopes, B-Lymphocyte/chemistry/immunology ; Erythrocytes/immunology/metabolism/parasitology ; Humans ; Kenya ; Malaria/*immunology/parasitology ; Malaria Vaccines/chemistry/immunology ; Membrane Proteins/chemistry/immunology ; Molecular Sequence Data ; Mutagenesis, Insertional/*genetics ; Plasmodium falciparum/*immunology ; Protein Structure, Tertiary/genetics ; Protozoan Proteins/chemistry/immunology ; Receptors, Immunologic/chemistry/genetics/*immunology/metabolism

1600-0536

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Introduction:  Cutaneous reactions due to use of non-steroidal anti-inflammatory drugs (NSAID) are frequently described. In the Contact Unit from the Department of Dermatology of Juan Canalejo Hospital, we compiled all cases studied during 4 years (from 2000 to 2003) to analyze the incidence, frequency and type of reactions derived from NSAIDs use.Material and methods:  We used a NAIDs battery (Aristegui lab.Ò) on 55 patients with clinical suspicion of adverse reaction from NSAIDs. We performed epicutaneous study on 17 and additional photoallergic study, after UVA irradiation (from 5 to 7,5 J/cm2), on 38 cases. In some cases we patched the drug used by patient as is and/or the active substance provided by the laboratory if it was not included on NSAIDs battery. Readings were done following ICDRG criteria at 48 and 96 hours on patch tests and 24 and 72 hours on photopatch tests. Healthy controls were patched.Results:  22 patients showed positive test: 15 cases were diagnosed as photoallergic dermatitis (etofenamate 5; dexketoprofen 4; ketoprofen 3; piroxicam 2; diclophenac 2 and aceclophenac 1). Five cases were explained as allergic contact dermatitis (etofenamate 2; fepradinol 2 and phenylbutazone 1) and two cases of fixed drug eruption from piroxicam were found.Conclusion:  In this study we observed that cutaneous reactions due to ketoprofen and piroxicam are still high. It is important to note the high incidence of allergic and photoallergic reactions from dexketoprofen, a recently introduced NSAID, reporting always a cross reaction between ketoprofen and dexketoprofen.

Electronic Resource

1600-0536

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Introduction:  Cutaneous adverse reactions from topical or systemic administration of non-steroidal anti-inflammatory drugs (NSAID) are frequently described. New cases of adverse reactions from NSAIDs are reported, but the most frequently involved are drugs as ketoprofen or piroxicam. We report a case of photoallergical contact dermatitis from diclophenac with possible cross reaction to aceclophenac.Material and methods:  A 63-year-old-man, allergic to penicillin, developed eczematous lesions on hands and face, after applying a medicament containing diclophenac. Patch test and photopatch test (5 J/cm2) were performed with a photoallergen battery (Chemotechnique diagnosticsÒ), a NSAIDs battery (Aristegui lab.Ò) and aceclophenac 1%, 5% and 10% in pet. Readings were done at 48 and 96 hours in epicutaneous test and at 24 and 72 hours in photoallergic study, following the ICDRG criteria.Results:  The results of photoallergic study were as follows: diclophenac 1%, 5% and 10% in pet. and aceclophenac 10% in pet. positive at 24 and 72 hours. The remaining compounds of the NSAIDs battery were negative.Conclusions:  Diclophenac is a NAIDs derived from aryl alcanoic acid group used both topically and systemically in Spain. Some adverse reactions have been reported as exanthema, urticaria, eczema or Steven-Johnson’s syndrome. We have found only two cases reported as photosensitization from diclophenac. In one of them, the clinical lesion was described as a granuloma annular-like and photoallergic study was negative. We report a photocontact dermatitis from diclophenac with positive photopatch test. Photopatch test positive to aceclophenac may be explained as a cross reaction among group.

Electronic Resource

1612-1112

Column liquid chromatography ; Phenolic Compounds ; Solvent extraction

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Summary The rate of extraction of phenolic compounds in two different solvents has been studied by liquid chromatography (HPLC) under reverse phase, gradient elution conditions. The solvents were diethyl ether and ethyl acetate. The method has been applied to two natural samples, a white wine and apple pulp.

Electronic Resource

1612-1112

Gel permeation chromatography ; Tannins in eucalyptus bark ; Polyphenols ; Molecular weight distribution

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Summary The molecular weight distribution of tannins from the wood, bark and leaves ofEucalyptus camaldulensis, E. globulus andE. rudis from two different Spanish provenances has been studied by high performance gel permeation chromatography, using the compounds' acetylated derivatives. The MW distribution profiles showed important variability depending on the type of vegetal tissue, the species and, in some cases, on the geographical provenance of the samples. Bark was the vegetal tissue that yielded tannins with the highest molecular weight, followed by wood and leaves. Tannins from wood and bark ofE. camaldulensis were of higher molecular size than those fromE. globulus andE. rudis; those in the leaves ofE. globulus andE. camaldulensis were similar in molecular size and larger than those in the leaves ofE. rudis.

Electronic Resource

1612-1112

Flavonols ; Capillary electrophoresis ; Temperature effects ; pH effects

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Summary The use of capillary electrophoresis for the analysis of selected flavonols present in fruit juices and wines (kaempferol-3-rutinoside, rutin, avicularin, quercitrin, isoquercitrin, isorhamnetin, kaempferol and quercetin) was explored, and the effect of pH and temperature on the separation studied. The method had good reproducibility and analyses were carried out in less than 10 minutes.

Electronic Resource

article

1997

Leuchtdiode ; Physik ; Plancksches Wirkungsquantum ; Quantenphysik

The Physics teacher, Bd. 35 (1997) H. 2, S. 108, 0031-921X

English

1089-7550

AIP Digital Archive

Physics

The correlation between the structural (average size and density) and optoelectronic properties [band gap and photoluminescence (PL)] of Si nanocrystals embedded in SiO2 is among the essential factors in understanding their emission mechanism. This correlation has been difficult to establish in the past due to the lack of reliable methods for measuring the size distribution of nanocrystals from electron microscopy, mainly because of the insufficient contrast between Si and SiO2. With this aim, we have recently developed a successful method for imaging Si nanocrystals in SiO2 matrices. This is done by using high-resolution electron microscopy in conjunction with conventional electron microscopy in dark field conditions. Then, by varying the time of annealing in a large time scale we have been able to track the nucleation, pure growth, and ripening stages of the nanocrystal population. The nucleation and pure growth stages are almost completed after a few minutes of annealing time at 1100 °C in N2 and afterward the ensemble undergoes an asymptotic ripening process. In contrast, the PL intensity steadily increases and reaches saturation after 3–4 h of annealing at 1100 °C. Forming gas postannealing considerably enhances the PL intensity but only for samples annealed previously in less time than that needed for PL saturation. The effects of forming gas are reversible and do not modify the spectral shape of the PL emission. The PL intensity shows at all times an inverse correlation with the amount of Pb paramagnetic centers at the Si–SiO2 nanocrystal–matrix interfaces, which have been measured by electron spin resonance. Consequently, the Pb centers or other centers associated with them are interfacial nonradiative channels for recombination and the emission yield largely depends on the interface passivation. We have correlated as well the average size of the nanocrystals with their optical band gap and PL emission energy. The band gap and emission energy shift to the blue as the nanocrystal size shrinks, in agreement with models based on quantum confinement. As a main result, we have found that the Stokes shift is independent of the average size of nanocrystals and has a constant value of 0.26±0.03 eV, which is almost twice the energy of the Si–O vibration. This finding suggests that among the possible channels for radiative recombination, the dominant one for Si nanocrystals embedded in SiO2 is a fundamental transition spatially located at the Si–SiO2 interface with the assistance of a local Si–O vibration. © 2002 American Institute of Physics.

Electronic Resource

1474-8673

Blackwell Publishing Journal Backfiles 1879-2005

Chemistry and Pharmacology

Medicine

1 Type 2 diabetes is associated with diverse oral pathologies in which salivary flow reduction is one of the causes of these oral abnormalities. Scarce literature exists regarding noradrenergic transmission and adrenergic-induced salivary flow in submaxillary and parotid glands of type 2 diabetic rats. 2 We studied noradrenergic transmission as well as the secretory response to α1- and β-adrenoceptor stimulation in the parotid and submaxillary glands of type 2 diabetic rats. 3 Diabetic rats exhibited diminished neuronal uptake, release and endogenous content of noradrenaline (NE) in both salivary glands. Further, NE synthesis was also diminished accompanied by decreased tyrosine hydroxylase activity. Salivary flow responses to α1-(methoxamine) and β-(isoprenaline) adrenoceptor stimulation were reduced in the submaxillary as well as the parotid glands of diabetic rats. 4 Our results suggest that the reduction of noradrenergic transmission in the salivary glands of type 2 diabetic rats is in part responsible for the diminished salivary flow evoked by α1- and β-adrenergic stimulation. Reduced noradrenergic activity may contribute to the pathophysiology of oral abnormalities in diabetic patients.

Electronic Resource

1089-7550

AIP Digital Archive

Physics

A reproducible method for the fabrication of ZnO transducers on InSb substrates is described. These transducers are being used for ultrasonic attenuation measurements over a wide low-frequency range (90–600 MHz) at very low temperatures(0.1–4.2 K). They have been characterized by their x-ray diffraction patterns, their insertion loss, and their contribution to the acoustic attenuation in InSb at low temperatures.

Electronic Resource

1365-3180

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

The effect of glyphosate on leaves and basal bulbs of yellow nutsedge (Cyperus esculentus L.) plants was studied. Treatment with glyphosate did not affect leaf anatomy, although light necrotic spots were seen on the veins. In contrast, basal bulb organization was greatly affected with large necrotic zones and disorganization in the vascular cylinder due to herbicide accumulation. Depletion or disappearance of vacuolar phenols was observed in bulbs of plants treated with the highest doses of glyphosate. The most remarkable effect of glyphosate was the appearance of one layer of sclerenchymatic cells between root and rhizome primordia and the cortical tissues which could play an important role in the inhibition of rhizome and root emergence by the herbicide.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background The role of Pyroglyphid mites in IgE-mediated serisitizalion has been well eslahlished. Although mites belonging to the genus Dermatophagoidess dominate in the acarofauna of domestic dust. non-pyroglyphid mites might also be of clinical importance. In Cuba, Dermatophagoides siboney is found in dust coexisting with D. pteronyssiuus and Blomia tropicalis. Dermatophagoides forinae is not found. Storage mites, such as Acorus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae might also be present.Objective The aim of this study was to investigate the allergenic relationships among the mites present in the environment of allergic patients in Cuba.Methods The crossreactivialty between an extract of D. siboney and the above mentioned six mites was studied by specific IgE inhibition, using sera of mite-sensitive patients after incubation with serial dilutions of D. sibaney.Results The inhibitory effect of D. sidiney extract was more pronounced against D. farinae. 91%, than against D. pyteronyssinus, 62%. Specific IgE to B. tropicali, A. siro, L. destructor and T. putrescentiae were inhibited to a much lesser extent, i.e. between 47 and 58%. The correlation between IgE to D. siboney and IgE to D. pteronyssinus and D. farinae was of 0.92 and 0.90. respectively. A weaker correlation was found between D. siboney and L. destructor (0.7), A. siro (0.67), B. tropicalis (0.54) and T. putrescentiae (0.51).Conclusion Thus. D. siboney seems to be most closely related to D. farinae.

Electronic Resource

0005-2760

(Mouse) ; Arachidonic acid ; Ethanol ; Icosanoid metabolism ; Macrophage ; Phospholipase A"2

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0006-291X

[abr] AA; arachidonic acid ; [abr] PAF; platelet-activating factor

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0022-3697

CdSe ; Debye temperature ; acousto-optic interaction ; elastic constants

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Physics

Electronic Resource