Search Results - (Author, Cooperation:A. Yoshimura)

2015-03-04

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adaptor Proteins, Signal Transducing/*metabolism ; Animals ; Body Weight ; Cell Differentiation ; Cell Proliferation ; Cytokine Receptor gp130/*metabolism ; Disease Models, Animal ; Enzyme Activation ; Epithelial Cells/*cytology/metabolism/pathology ; HEK293 Cells ; Homeostasis ; Humans ; Inflammation/*metabolism/pathology ; Inflammatory Bowel Diseases/metabolism/pathology ; Intestinal Mucosa/*cytology/metabolism/pathology ; Mice ; Phosphoproteins/*metabolism ; Proto-Oncogene Proteins c-yes/metabolism ; Proto-Oncogene Proteins pp60(c-src)/*metabolism ; Receptors, Notch/metabolism ; *Regeneration ; Signal Transduction ; Up-Regulation

2013-03-12

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amyotrophic Lateral Sclerosis ; Animals ; Animals, Newborn ; Axons/metabolism/pathology ; Cell Death ; Diaphragm/innervation ; Embryo Loss ; Embryo, Mammalian/metabolism/pathology ; Exons/genetics ; Female ; Fibroblasts ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Motor Neurons/*metabolism/*pathology ; Muscular Atrophy, Spinal ; Neuromuscular Diseases/metabolism/pathology ; Oxidative Stress ; RNA Processing, Post-Transcriptional ; RNA, Transfer, Tyr/genetics/*metabolism ; Respiration ; Spinal Nerves/cytology ; Transcription Factors/deficiency/*metabolism ; Tumor Suppressor Protein p53/metabolism ; Tyrosine/genetics/metabolism

2018-08-07

Rockefeller University Press

0022-1007

1540-9538

Medicine

Innate Immunity and Inflammation

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

The brown planthopper (BPH) is one of the most destructive insect pests of rice. Resistant varieties have proved to be one of the most economic and effective measures for BPH management. In this study, an indica rice ‘DV85’ showed resistance to biotype 2 of BPH by bulked seedling test, and a recombinant inbred line (RIL) population derived from a cross between a susceptible rice ‘Kinmaze’ and ‘DV85’ was phenotyped to map genetic factors conferring BPH resistance in ‘DV85′. Composite interval mapping revealed that one quantitative trait locus (QTL) with a LOD score of 10.1 was detected between XNpb202 and C1172 on chromosome 11. This QTL was designated as Qbph11. Qbph11 explained 68.4% of the phenotypic variance of BPH resistance in this population. The allele from the resistant parent ‘DV85’ at Qbph11 reduced the damage caused by BPH feeding and would be very useful in breeding resistant rice varieties via marker-assisted selection.

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Complement-dependent cytotoxicity (CDC) mediated by a chimeric anti-Lewis Y monoclonal antibody (cH18A; human IgGl) was investigated in this study. Human lung adenocarcinoma cell lines (PC7, PC9, and PC 14) were used as the target cells. PC7 and PC9 cells, expressed Lewis Y antigen and were lysed by cH18A as effectively as by the parent mouse anti-Lewis Y antibodies (mH18A) in a concentration-dependent manner. PC14 cells did not express Lewis Y antigen and were not lysed by either cH18A or mH18A. cH18A mediated CDC activity against PC7 and PC9 cells was enhanced by the combined use of monoclonal antibodies directed against CD46(MCP), CD55(DAF), and CD59. These molecules are complement-regulatory proteins which protect host cells from CDC. PC7 and PC9 cells, showed high levels of surface expression of these proteins, PC7 cells were more susceptible to cH ISA-mediated CDC than PC9 cells. Use of multiple blocking antibodies to the complement-regulatory proteins produced more enhancement of cH18A-mediated CDC than a single antibody. Moreover, expression of CD55 and CD59 by PC7 and PC9 cells was decreased after treatment with Pl-PLC, resulting in increased susceptibility to cHISA-mediated CDC. Although the reason is unknown, PC7 cells became more susceptible to CDC than PC9 cells after PI-PLC treatment even in the absence of cH18A. These data suggest that chimeric monoclonal antibodies can be used to induce CDC against lung adenocarcinoma, and that such CDC is potentiated by a variety of antibodies blocking compliment-regulatory proteins on the tumour cell surface.

Electronic Resource

0005-2736

(Chinese hamster cell) ; Amphotericin B ; Cholesterol synthesis ; Membrane fluidity ; Membrane fusion ; Sendai virus

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0020-1693

Charge transfer excited state ; Dinuclear complexes ; Mixed-valence complexes ; Ruthenium complexes ; Spectroelectrochemistry

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0014-4827

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Medicine

Electronic Resource

0031-9163

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0010-8545

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

1010-6030

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0304-4165

(Human) ; (KB cell) ; Multidrug resistance ; P-glycoprotein

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0304-4165

(Human tissue) ; (KB cell) ; Glycosylation ; Multidrug resistance ; P-glycoprotein

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0304-4165

(Human) ; Immunoassay ; Thymidine phophorylase ; Tissue distribution

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0304-4165

(Adrenal gland) ; Glycoprotein ; Multidrug resistance ; P-glycorprotein ; Progesterone ; Progesterone metabolite ; Transport activity

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0370-2693

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Ganglioside expression on cytotoxic T lymphocytes induced against the mouse erythroleukaemia line FBL-3N, was analysed, compared with that of naive T lymphocytes in the thymus, lymph nodes and spleen. Although normal uncultured and cultured T lymphocytes expressed no GD2, GD3 or GM2 gangliosides, cytotoxic T cells with CD4+ CD8−, CD4−CD8+, and CD4−CD8− phenotypes reacted with anti-GD2 monoclonal antibody with various intensities. The reactivity with anti-GD2 was associated with the intensity of cytotoxic activity as analysed using cytotoxic T lymphocyte (CTL) clones established from the bulk CTLs of each phenotype. An increase of the mRNA expression of GM2/GD2 synthase gene was demonstrated by Northern blot hybridization using RNA extracted from thymocytes, spleen cells, Con A-treated spleen cells and various types of CTL cells. These results indicated that GD2 ganglioside expression might be associated with the functional differentiation of murine T lymphocytes.

Electronic Resource

1600-0765

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Polymorphonuclear leukocytes (PMN) are the first cells that migrate into periodontal tissues and gingival crevices in response to invading pathogens. It was recently demonstrated that PMN have the ability to synthesize and release cytokines following appropriate stimulation, while it is not clear whether these capacities are directly related to periodontal destructive processes. We therefore investigated the amounts of the cytokines interleukin-1β (IL-lβ), tumor necrosis factor α (TNF-α), IL-8 and IL-1 receptor antagonist (IL-lra) secreted by PMN from healthy donors following stimulation with lipopolysaccharide (LPS) from 4 periodontopathic bacteria, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Capnocytophaga ochracea and Fusobacterium nucleatum, and the non-oral bacterium Escherichia coli. A. actinomycetemcomitans, F. nucleatum and E. coli LPS stimulated the release of significantly greater amounts of IL-lβ, TNF-α and IL-8 than the control unstimulated PMN (p〈0.01). The levels of IL-lβ, TNF-α and IL-8 released from cells stimulated with P. gingivalis or C. ochracea LPS were significantly lower than those of cells stimulated with A. actinomycetemcomitans or E. coli LPS (p〈0.05). On the other hand, substantially greater amounts of IL-lra were released from PMN stimulated with each LPS and from control unstimulated PMN during the first 6 h, and then significantly greater amounts of IL-lra were secreted by PMN stimulated with A. actinomycetemcomitans and E.coli LPS during the following 12 h (p〈0.01). The inhibitory effects of IL-lra on the biological activity of IL-1 in the supernatants of PMN were examined by the thymocyte comitogen proliferation assay. The supernatants of PMN stimulated with each LPS showed less biological IL-1 activity as compared with the same doses of recombinant human IL-lβ detected by enzyme-linked immunosorbent assay. Furthermore, no activity was detected in the supernatants of PMN stimulated with P. gingivalis or C. ochracea LPS. These findings demonstrated that LPS from periodontopathic bacteria were capable of stimulating PMN to release not only pro-inflammatory cytokines but also their inhibitors such as IL-lra. Different secretion levels of these cytokines and their biological activities induced by the various LPS might be important in the onset and progression of periodontal diseases.

Electronic Resource

1435-1528

ElasticBingham fluid ; yieldstress ; oscillatoryshear ; oil-in-wateremulsion ; nonlinear rheological behavior

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Physics

Abstract The response of an elastic Bingham fluid to oscillatory strain has been modeled and compared with experiments on an oil-in-water emulsion. The newly developed model includes elastic solid deformation below the yield stress (or strain), and Newtonian flow above the yield stress. In sinusoidal oscillatory deformations at low strain amplitudes the stress response is sinusoidal and in phase with the strain. At large strain amplitudes, above the yield stress, the stress response is non-linear and is out of phase with strain because of the storage and release of elastic recoverable strain. In oscillatory deformation between parallel disks the non-uniform strain in the radial direction causes the location of the yield surface to move in-and-out during each oscillation. The radial location of the yield surface is calculated and the resulting torque on the stationary disk is determined. Torque waveforms are calculated for various strains and frequencies and compared to experiments on a model oil-in-water emulsion. Model parameters are evaluated independently: the elastic modulus of the emulsion is determined from data at low strains, the yield strain is determined from the phase shift between torque and strain, and the Bingham viscosity is determined from the frequency dependence of the torque at high strains. Using these parameters the torque waveforms are predicted quantitatively for all strains and frequencies. In accord with the model predictions the phase shift is found to depend on strain but to be independent of frequency.

Electronic Resource