Search Results - (Author, Cooperation:A. Y. Lee)

2014-04-12

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Cell Line, Tumor ; Cells/*drug effects ; Drug Evaluation, Preclinical/*methods ; Drug Resistance/*genetics ; *Gene Regulatory Networks ; Genome-Wide Association Study/*methods ; Haploinsufficiency ; Humans ; Pharmacogenetics ; Saccharomyces cerevisiae/drug effects/genetics ; Small Molecule Libraries/*pharmacology

2015-02-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Alleles ; Allelic Imbalance/genetics ; *Cell Differentiation/genetics ; Cell Lineage/genetics ; Chromatin/*chemistry/genetics/*metabolism ; *Chromatin Assembly and Disassembly/genetics ; Embryonic Stem Cells/*cytology/*metabolism ; Enhancer Elements, Genetic/genetics ; Epigenesis, Genetic/*genetics ; Epigenomics ; Gene Regulatory Networks ; Humans ; Promoter Regions, Genetic/genetics ; Reproducibility of Results

2015-02-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Acetylation ; *Alleles ; Chromatin/genetics/metabolism ; Chromosomes, Human/genetics ; Datasets as Topic ; Enhancer Elements, Genetic/genetics ; Epigenesis, Genetic/*genetics ; *Epigenomics ; Genetic Variation/genetics ; Haplotypes/*genetics ; Histones/metabolism ; Humans ; Nucleotide Motifs ; Organ Specificity/genetics ; Transcription, Genetic/genetics

2013-10-22

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Cell Line ; Chromatin/chemistry/genetics/*metabolism ; *Chromosome Mapping ; Enhancer Elements, Genetic/physiology ; Gene Expression Regulation ; *Genome, Human ; Humans ; Imaging, Three-Dimensional ; Promoter Regions, Genetic/physiology ; Protein Binding ; Signal Transduction ; Tumor Necrosis Factor-alpha/metabolism

1600-0560

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

An electron microscopic study was made of a patient with erythema elevatum diutinum, a rare variant of cutaneous leukocytoclastic vasculitis. Biopsies were obtained from early evolving and late fibroproliferative lesions. Early lesions showed vasculitis and a massive dermal infiltrate composed mainly of neutrophils, histiocytes/macrophages and apparently increased Langerhans cells. Later lesions were characterized by a dense fibrosis with a proliferation of fibroblasts and myofibroblasts and a dermal infiltrate in which lymphocytes and histiocytes/macrophages predominated. Even in later lesions, dermal Langerhans cells were observed in great number. Langerhans cells may be involved in the evolution of this rare disease.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of pre- and post-treatment with naloxone on the cardiotoxicity of ouabain in the guinea-pig were studied.2. After pretreatment with naloxone, the dose of ouabain required to induce ventricular arrhythmias and cardiac arrest were significantly increased, in a dose-dependent manner, compared with the control, indicating a protective effect of naloxone against digitalis intoxication.3. Administration of naloxone at the onset of cardiac arrhythmias induced by a lethal dose of ouabain restored the cardiac rhythm and consequently saved life in seven out of eight animals, indicating an antiarrhythmic effect of naloxone in digitalis-intoxicated guinea-pigs.4. The protective and antiarrhythmic effects of naloxone against digitalis intoxication have clinical implications.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of myocardial ischaemia and reperfusion on arrhythmias and cAMP levels were studied using the Langendoff isolated perfused rat heart preparation.2. Myocardial ischaemia and reperfusion caused arrhythmias and augmentation of cAMP levels concurrently, supporting the suggestion that myocardial cAMP is related to arrhythmias.3. Pretreatment with naloxone attenuated both arrhythmias and augmentation of cAMP levels to a similar extent. The results suggest that the anti-arrhythmic effect of naloxone may involve myocardial cAMP.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of naloxone on contractility and cardiac rhythm were studied in the rat isolated perfused heart during myocardial ischaemia and reperfusion.2. Pretreatment of the rat isolated perfused heart with naloxone abolished the reduction in left ventricular pressures and attenuated greatly the arrhythmias due to myocardial ischaemia and reperfusion.3. Administration of naloxone into the fibrillating rat isolated heart induced by myocardial ischaemia and reperfusion also attenuated the arrhythmias in a dose-dependent manner.4. The results indicate a possible involvement of the endogenous opioid peptides in the cardiac effects due to myocardial ischaemia and reperfusion. The anti-arrhythmic effect of naloxone has great clinical implications.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of reserpine treatment on the myocardial contents of catecholamines and enkephalins and the incidence of ventricular arrhythmias during ischaemia and reperfusion in the isolated rat heart were studied.2. Reserpine treatment almost completely depleted the heart of noradrenaline (NA). It also significantly depleted the heart of adrenaline and dopamines. It did not, however, alter the myocardial contents of enkephalins.3. Reserpine-treatment attenuated significantly, but did not abolish, cardiac arrhythmias induced by ischaemia and reperfusion in the isolated heart preparation.4. The results of the present study indicate that myocardial catecholamines especially NA are a contributing factor to arrhythmogenesis during ischaemia and reperfusion.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of naloxone and propranolol on cardiac arrhythmias and durations from respiratory arrest to ventricular asystole and cardiac standstill were studied in unanaesthetized young rats induced to suffer respiratory arrest and exhibit ventricular fibrillation (VF) by a modified chloroform hypoxia technique.2. Both naloxone and propranolol reduced the incidence of VF dose dependently, indicating that they have antiarrhythmic effects. Addition of naloxone to propranolol shifted the dose-response curve of propranolol to the left significantly, indicating an additive effect of the two drugs in their antiarrhythmic activity.3. Both naloxone and propranolol prolonged the duration from respiratory arrest to ventricular asystole. However, joint administration of both did not further prolong this duration indicating an absence of additive effects.4. Naloxone prolonged the duration from respiratory arrest to cardiac standstill, indicating that naloxone prolonged the survival time. In contrast, propranolol did not produce the same effect.5. That naloxone both produced antiarrhythmic effect and prolonged the survival time whereas propranolol only corrected cardiac arrhythmias suggests that the antiarrhythmic effect of naloxone may not result in prolongation of survival time and that different mechanisms may be involved in the antiarrhythmic effect.

Electronic Resource

1440-1681

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

1. The effects of intracerebroventricular (i.c.v.) and intracisternal (i.e.) injection of β-endorphin on arterial blood pressure (BP) in rats that received five intraperitoneal injections of monosodium glutamate (MSG) on alternate days in the first 10 days of life were studied.2. β-endorphin administered into the lateral ventricles caused a prolonged elevation in BP, whereas i.c. injection of the peptide resulted in an even longer lasting reduction in BP. In the MSG-treated rat, the prolonged hypertensive effect of i.c.v. injection of β-endorphin was completely abolished, but the effect of i.c. injection of the peptide was the same as that in the control. Since MSG treatment destroyed selectively the structures around the third ventricle, it is suggested that these structures, including the arcuate nucleus, may be responsible for mediating the cardiovascular effects of β-endorphin.3. The effects of central administration of β-endorphin were completely blocked by naloxone, which mainly antagonizes the actions of μ-receptor agonists and has no cardiovascular effects itself. The results suggest that μ-receptors may be involved in mediation of the effects of β-endorphin on the cardiovascular system and that β-endorphin in the brain may not exert a tonic influence on the cardiovascular functions.

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background  Vitiligo may develop following minor physical trauma. However, in autologous epidermal grafting, depigmentation of the donor (normally pigmented) site from a suction blister is rare, even in cases displaying failure of repigmentation at the recipient (depigmented) site.Objectives  To examine whether the suction procedure is more likely to damage keratinocytes in the depigmented than in the normally pigmented epidermis of vitiligo, and to determine what kind of damage occurs to the keratinocytes.Methods  Paired roofs of suction blisters from five patients with generalized vitiligo, five with localized and seven with segmental type, were used for the study. Multiple new lesions developed in two of the five patients with the generalized type. Apoptosis of keratinocytes in the epidermis was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-digoxigenin nick end labelling (TUNEL) staining, with immunohistochemistry for Bax and active caspase 3. Expression of Bcl-2, Bax, FLIP and p53, activation of caspases 3, 8 and 9, and cleavage of poly(adenosine diphosphate ribose) polymerase (PARP) in the epidermis were analysed by Western blotting in four patients with each type.Results  Apoptotic keratinocytes, which stained with TUNEL and anti-Bax and antiactive caspase 3 antibodies, were scattered in the blistered epidermis, mainly in the lower portions. The depigmented epidermis displayed significantly more apoptotic keratinocytes than the normally pigmented epidermis. The numerical difference between the paired epidermides was related to the disease activity and not to the type of lesions. The number of apoptotic keratinocytes in the normally pigmented epidermis was as high as that in the depigmented epidermis in the two patients with active generalized type vitiligo. Expression of Bax and p53 in the depigmented epidermis was higher than in the normally pigmented epidermis, whereas expression of FLIP was lower. In addition, the activation of caspases 3, 8 and 9, and cleavage of PARP, were increased in the depigmented compared with the normally pigmented epidermis. The degree of difference in expression and activation was parallel to the results of the TUNEL assay.Conclusions  The keratinocytes in the depigmented compared with the normally pigmented epidermis of vitiligo may become apoptotic more easily after suction.

Electronic Resource

0014-4827

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Medicine

Electronic Resource

1439-0973

Springer Online Journal Archives 1860-2000

Medicine

Zusammenfassung Fusarium wird im allgemeinen als opportunistischer Pilz beim Menschen angesehen. Im Fuß einer Patientin ohne Abwehrschwäche trat eine abszedierende Infektion durchFusarium solani auf, die sogar nach intravenöser Amphotericin B-Therapie rezidivierte.

Summary Fusarium spp. are usually considered opportunistic fungi in humans. A case ofFusarium solani abscess formation of the foot in an immunocompetent patient in whom recurrence occurred even after intravenous amphotericin B treatment is presented here.

Electronic Resource

1573-2878

Padé approximation ; hereditary optimal control problems ; nonlinear time-lag systems ; computational schemes ; two-sided Laplace transform ; gradient algorithms

Springer Online Journal Archives 1860-2000

Mathematics

Abstract In this paper, we consider a particular approximation scheme which can be used to solve hereditary optimal control problems. These problems are characterized by variables with a time-delayed argumentx(t − τ). In our approximation scheme, we first replace the variable with an augmented statey(t) ≜x(t - τ). The two-sided Laplace transform ofy(t) is a product of the Laplace transform ofx(t) and an exponential factor. This factor is approximated by a first-order Padé approximation, and a differential relation fory(t) can be found. The transformed problem, without any time-delayed argument, can then be solved using a gradient algorithm in the usual way. Four problems are solved to illustrate the validity and usefulness of this technique.

Electronic Resource

1573-2878

Sequential gradient-restoration algorithm ; nonlinear optimization problems ; bounded terminal conditions ; Valentine's device

Springer Online Journal Archives 1860-2000

Mathematics

Abstract Bounded terminal conditions of nonlinear optimization problems are converted to equality terminal conditions via the Valentine's device. In so doing, additional unknown parameters are introduced into the problem. The transformed problems can still be easily solved using the sequential gradient-restoration algorithm (SGRA) via a simple augmentation of the unknown parameter vector π. Three example problems with bounded terminal conditions are solved to verify this technique.

Electronic Resource

1573-2878

Sequential gradient-restoration algorithm ; backward sweep method ; path equality constraints ; second variation ; neighboring optimal feedback laws

Springer Online Journal Archives 1860-2000

Mathematics

Abstract Neighboring extremals of dynamic optimization problems with path equality constraints and with an unknown parameter vector are considered in this paper. With some simplifications, the problem is reduced to solving a linear, time-varying two-point boundary-value problem with integral path equality constraints. A modified backward sweep method is used to solve this problem. Two example problems are solved to illustrate the validity and usefulness of the solution technique.

Electronic Resource