Search Results - (Author, Cooperation:A. Salas)

Showing 1 - 20 results of 21, query time: 0.29s Refine Results
  1. 1
    I. Lazaridis ; N. Patterson ; A. Mittnik ; G. Renaud ; S. Mallick ; K. Kirsanow ; P. H. Sudmant ; J. G. Schraiber ; S. Castellano ; M. Lipson ; B. Berger ; C. Economou ; R. Bollongino ; Q. Fu ; K. I. Bos ; S. Nordenfelt ; H. Li ; C. de Filippo ; K. Prufer ; S. Sawyer ; C. Posth ; W. Haak ; F. Hallgren ; E. Fornander ; N. Rohland ; D. Delsate ; M. Francken ; J. M. Guinet ; J. Wahl ; G. Ayodo ; H. A. Babiker ; G. Bailliet ; E. Balanovska ; O. Balanovsky ; R. Barrantes ; G. Bedoya ; H. Ben-Ami ; J. Bene ; F. Berrada ; C. M. Bravi ; F. Brisighelli ; G. B. Busby ; F. Cali ; M. Churnosov ; D. E. Cole ; D. Corach ; L. Damba ; G. van Driem ; S. Dryomov ; J. M. Dugoujon ; S. A. Fedorova ; I. Gallego Romero ; M. Gubina ; M. Hammer ; B. M. Henn ; T. Hervig ; U. Hodoglugil ; A. R. Jha ; S. Karachanak-Yankova ; R. Khusainova ; E. Khusnutdinova ; R. Kittles ; T. Kivisild ; W. Klitz ; V. Kucinskas ; A. Kushniarevich ; L. Laredj ; S. Litvinov ; T. Loukidis ; R. W. Mahley ; B. Melegh ; E. Metspalu ; J. Molina ; J. Mountain ; K. Nakkalajarvi ; D. Nesheva ; T. Nyambo ; L. Osipova ; J. Parik ; F. Platonov ; O. Posukh ; V. Romano ; F. Rothhammer ; I. Rudan ; R. Ruizbakiev ; H. Sahakyan ; A. Sajantila ; A. Salas ; E. B. Starikovskaya ; A. Tarekegn ; D. Toncheva ; S. Turdikulova ; I. Uktveryte ; O. Utevska ; R. Vasquez ; M. Villena ; M. Voevoda ; C. A. Winkler ; L. Yepiskoposyan ; P. Zalloua ; T. Zemunik ; A. Cooper ; C. Capelli ; M. G. Thomas ; A. Ruiz-Linares ; S. A. Tishkoff ; L. Singh ; K. Thangaraj ; R. Villems ; D. Comas ; R. Sukernik ; M. Metspalu ; M. Meyer ; E. E. Eichler ; J. Burger ; M. Slatkin ; S. Paabo ; J. Kelso ; D. Reich ; J. Krause
    Nature Publishing Group (NPG)
    Published 2014
    Staff View
    Publication Date:
    2014-09-19
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Agriculture/history/manpower ; Asia/ethnology ; Europe ; European Continental Ancestry Group/*classification/*genetics ; Genome, Human/*genetics ; History, Ancient ; Humans ; Population Dynamics ; Principal Component Analysis
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    D. Reich ; N. Patterson ; D. Campbell ; A. Tandon ; S. Mazieres ; N. Ray ; M. V. Parra ; W. Rojas ; C. Duque ; N. Mesa ; L. F. Garcia ; O. Triana ; S. Blair ; A. Maestre ; J. C. Dib ; C. M. Bravi ; G. Bailliet ; D. Corach ; T. Hunemeier ; M. C. Bortolini ; F. M. Salzano ; M. L. Petzl-Erler ; V. Acuna-Alonzo ; C. Aguilar-Salinas ; S. Canizales-Quinteros ; T. Tusie-Luna ; L. Riba ; M. Rodriguez-Cruz ; M. Lopez-Alarcon ; R. Coral-Vazquez ; T. Canto-Cetina ; I. Silva-Zolezzi ; J. C. Fernandez-Lopez ; A. V. Contreras ; G. Jimenez-Sanchez ; M. J. Gomez-Vazquez ; J. Molina ; A. Carracedo ; A. Salas ; C. Gallo ; G. Poletti ; D. B. Witonsky ; G. Alkorta-Aranburu ; R. I. Sukernik ; L. Osipova ; S. A. Fedorova ; R. Vasquez ; M. Villena ; C. Moreau ; R. Barrantes ; D. Pauls ; L. Excoffier ; G. Bedoya ; F. Rothhammer ; J. M. Dugoujon ; G. Larrouy ; W. Klitz ; D. Labuda ; J. Kidd ; K. Kidd ; A. Di Rienzo ; N. B. Freimer ; A. L. Price ; A. Ruiz-Linares
    Nature Publishing Group (NPG)
    Published 2012
    Staff View
    Publication Date:
    2012-07-18
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Americas ; Asia ; Cluster Analysis ; Emigration and Immigration/*history/statistics & numerical data ; Gene Flow ; Genetics, Population ; History, Ancient ; Humans ; Indians, North American/*genetics/*history ; Models, Genetic ; *Phylogeny ; Polymorphism, Single Nucleotide/genetics ; Siberia
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Moreno-Mayar, J. V., Vinner, L., de Barros Damgaard, P., de la Fuente, C., Chan, J., Spence, J. P., Allentoft, M. E., Vimala, T., Racimo, F., Pinotti, T., Rasmussen, S., Margaryan, A., Iraeta Orbegozo, M., Mylopotamitaki, D., Wooller, M., Bataille, C., Becerra-Valdivia, L., Chivall, D., Comeskey, D., Deviese, T., Grayson, D. K., George, L., Harry, H., Alexandersen, V., Primeau, C., Erlandson, J., Rodrigues-Carvalho, C., Reis, S., Bastos, M. Q. R., Cybulski, J., Vullo, C., Morello, F., Vilar, M., Wells, S., Gregersen, K., Hansen, K. L., Lynnerup, N., Mirazon Lahr, M., Kjaer, K., Strauss, A., Alfonso-Durruty, M., Salas, A., Schroeder, H., Higham, T., Malhi, R. S., Rasic, J. T., Souza, L., Santos, F. R., Malaspinas, A.-S., Sikora, M., Nielsen, R., Song, Y. S., Meltzer, D. J., Willerslev, E.
    American Association for the Advancement of Science (AAAS)
    Published 2018
    Staff View
    Publication Date:
    2018-12-07
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Anthropology, Genetics, Online Only
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Tarroch, X. ; Vives, P. ; Salas, A. ; Moré, J.

    Copenhagen : Munksgaard International Publishers
    Published 2001
    Staff View
    ISSN:
    1600-0560
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Background: Cutaneous findings other than hyperpigmentation are rare in Whipple’s disease.Case report: We present the case of a 59-year-old man previously diagnosed with Whipple’s disease by duodenal biopsy, who developed red-brown, painful, subcutaneous nodules on the buttocks, thighs, arms and legs. Biopsy of these nodules showed a septal panniculitis and foamy macrophages containing PAS-positive, diastase resistant intracytoplasmic material, characteristic of Whipple’s disease and similar to that observed in the duodenal biopsy. Ultrastructurally, this material in the histiocytes corresponded to degenerated bacilli.Conclusions: This is the fourth documented case of subcutaneous involvement by Whipple’s disease. One should consider the possibility of Whipple’s disease in any patient who presents with symptoms compatible with that condition who demonstrates septal panniculitis with a large amount of foamy histiocytes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1034/j.1600-0560.2001.280706.x

    Articles: DFG German National Licenses
  5. 5
    Ortega-Salas, A. A.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1980
    Staff View
    ISSN:
    1095-8649
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Otolith accretion was greatest in summer when opaque bands were laid down and slowest in winter when hyaline rings were formed. Food intake was minimal in winter and maximal in summer. Liver weight was high in summer and winter and low in spring after spawning. Growth in length was higher in summer than winter. Gonad weight was greatest in winter for both sexes. Spawning occurred from February to April. After spawning males recovered earlier than females and testes ripened earlier than the ovaries. From winter to summer, the sea temperature increased from 7.5–13.0°C; the day length from 7–17h; and the salinity varied from 34.07−34.36%.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1095-8649.1980.tb03687.x

    Articles: DFG German National Licenses
  6. 6
    Garry, G. ; Forbes, G. A. ; Salas, A. ; Santa Cruz, M. ; Perez, W. G. ; Nelson, R. J.

    Oxford, UK : Blackwell Science Ltd
    Published 2005
    Staff View
    ISSN:
    1365-3059
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    To test the hypothesis that isolates of Phytophthora infestans attacking wild Solanaceae exhibit specialization for particular host species, 115 isolates of P. infestans were collected from cultivated potatoes, nontuber-bearing Solanum spp. of the Basarthrum section and wild tomatoes from five departments in the northern and central highlands of Peru, and characterized using several neutral markers. All isolates belonged to one of four clonal lineages described previously in Peru: EC-1, US-1, PE-3 and PE-7. There was a strong association of three lineages with host species: PE-3 was only isolated from cultivated potato, while PE-7 and US-1 were only isolated from nontuber-bearing Solanum spp. (Basarthrum section and wild tomatoes). EC-1 was isolated from all host groups sampled. A subset (n = 74) of the isolates was evaluated for metalaxyl resistance. High levels of resistance were found almost exclusively in EC-1 and PE-3, while US-1 and PE-7 isolates were generally sensitive. In a detached-leaf assay for lesion diameter using five EC-1 isolates from S. caripense and seven EC-1 isolates from cultivated potato, there was a significant interaction between isolate origin and inoculated host, caused by higher aggressiveness of EC-1 from cultivated potato on its host of origin. In a comparison of EC-1 (seven isolates from cultivated potato) and US-1 (three isolates from S. caripense), each pathogen lineage was more aggressive on its original host species, causing a highly significant interaction between isolate origin and inoculated host. Wild tomatoes and nontuber-bearing Solanum spp. harbour several pathogen lineages in Peru and could serve as reservoirs of inoculum that might contribute to epidemics on potato or tomato. Potential risks associated with the use of wild Solanum hosts as sources of resistance to P. infestans are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1365-3059.2005.01250.x

    Articles: DFG German National Licenses
  7. 7
    Salas, A ; Fernández-Bañares, F ; Casalots, J ; González, C ; Tarroch, X ; Forcada, P ; González, G

    Oxford, UK : Blackwell Science Ltd
    Published 2003
    Staff View
    ISSN:
    1365-2559
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Aims:  To assess differences in the pattern of subepithelial myofibroblasts and the expression of tenascin as a marker of extracellular matrix production in collagenous and lymphocytic colitis.Methods and results:  Colorectal biopsies were studied from 122 patients with chronic diarrhoea and normal colonoscopy. The pathological diagnoses were collagenous colitis (n = 35), lymphocytic colitis (n = 37), mild non-specific chronic inflammation (n = 28) and normal mucosa (n = 18). Four cases showed features of collagenous colitis but with collagen bands 〈10 µm thick. Normal mucosa from 14 patients without diarrhoea served as healthy control tissue. Immunohistochemical expression of α-smooth muscle actin (myofibroblast marker) and tenascin was evaluated in well-orientated sections. The expression of α-smooth muscle actin was significantly increased in collagenous colitis compared with all the other groups. Strong tenascin subepithelial expression was seen in all cases of collagenous colitis, including the four without full-blown features. The mean thickness of tenascin bands was greater than that obtained by conventional stains.Conclusions:  There are clear differences, with respect to extracellular matrix remodelling, between collagenous and lymphocytic colitis. These results support the theory of matrix overproduction in the genesis of collagenous colitis.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1365-2559.2003.01650.x

    Articles: DFG German National Licenses
  8. 8
    Gomez-Beltran, F. ; Salas, A. ; Valero, A.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0003-2670
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(01)84045-9

    Articles: DFG German National Licenses
  9. 9
    Greenside, H.S. ; Reiman, A.H. ; Salas, A.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0021-9991
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Computer Science
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0021-9991(89)90066-1

    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    0010-4655
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Computer Science
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0010-4655(93)90031-7

    Articles: DFG German National Licenses
  11. 11
    Viana, M.T. ; Lopez, L.M. ; Salas, A.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0044-8486
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0044-8486(93)90131-H

    Articles: DFG German National Licenses
  12. 12
    Staff View
    ISSN:
    1749-6632
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Natural Sciences in General
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1749-6632.1976.tb49909.x

    Articles: DFG German National Licenses
  13. 13
    Staff View
    ISSN:
    1534-4681
    Keywords:
    Mammography ; Breast cancer ; Local recurrence ; Ductal carcinoma in situ ; Skin-sparing mastectomy
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract Background: Skin-sparing mastectomy with immediate transverse rectus abdominis musculocutaneous (TRAM) flap reconstruction is being used more often for the treatment of breast cancer. Mammography is not used routinely to evaluate TRAM flaps in women who have undergone mastectomy. We have identified the potential value of its use in selected patients. Methods and Results: We report on four women who manifested local recurrences in TRAM flaps after initial treatment for ductal carcinoma in situ (DCIS) or DCIS with microinvasion undergoing skin-sparing mastectomy and immediate reconstruction. All four patients presented with extensive, high-grade, multifocal DCIS that precluded breast conservation. Three of four mastectomy specimens demonstrated tumor close to the surgical margin. Three of the four recurrences were detected by physical examination; the remaining local recurrence was documented by screening mammography. The recurrences had features suggestive of malignancy on mammography. Conclusion: We conclude that all patients undergoing mastectomy and TRAM reconstruction for extensive, multifocal DCIS should undergo regular routine mammography of the reconstructed breast. Our experience with this subgroup of patients raises concern about the value of skin-sparing mastectomy with immediate reconstruction for therapy. Adjuvant radiation therapy should be recommended for those patients with negative but close surgical margins.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF02303866

    Articles: DFG German National Licenses
  14. 14
    Barral, S. ; Lareu, M. V. ; Salas, A. ; Carracedo, A.
    Springer
    Published 2000
    Staff View
    ISSN:
    1437-1596
    Keywords:
    Key words D22S683 ; D6S477 ; Short tandem repeats ; Sequence structure ; DNA polymorphisms
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Law
    Notes:
    Abstract For two short tandem repeats at the D22S683 and D6S477 loci, 30 and 22 selected alleles, respectively were sequenced. A total of 20 different alleles were found for the D22S683 locus and 12 alleles for the D6S477 locus. In both systems the alleles were designated according to the total number of repeats. D22S683 is a hypervariable STR consisting of blocks of (TATC) repeats with a basic sequence structure (TATATC)n (TATC)n (ATC)0–1 (TATC)n. The D6S477 locus consists of blocks of (TCTA) repeats with a basic sequence structure (TCTA)n (TA)1 (TCTA)0–2 (TA)0–1 (TCTA)n. Population data showed a heterozygosity of 0.89 for D22S683 and 0.75 for D6S477. These STRs are promising markers for forensic genetics as they are robust and can be easily included in multiplexes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s004140050286

    Articles: DFG German National Licenses
  15. 15
    Lareu, M. V. ; Barral, S. ; Salas, A. ; Carracedo, A.
    Springer
    Published 1998
    Staff View
    ISSN:
    1437-1596
    Keywords:
    Key words Short tandem repeats ; Human genome ; DNA sequencing ; DNA polymorphisms
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Law
    Notes:
    Abstract A short tandem repeat in the D18S535 locus was sequenced in 25 selected alleles. A total of 8 different alleles were found which can be designated according to the total number of repeats. This STR is a simple hypervariable STR consisting of blocks of (GATA) repeats with a basic sequence structure (GATA)1(GACA)1(GATA)1 (GAT)1(GATA)9–16. Population data showed that this is a highly polymorphic STR with a heterozygosity of more than 0.80, a simple structure and small size (130–158 bp) which makes this an interesting DNA polymorphism for forensic and genetic purposes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s004140050185

    Articles: DFG German National Licenses
  16. 16
    Staff View
    ISSN:
    0090-6980
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0090-6980(89)90063-4

    Articles: DFG German National Licenses
  17. 17
    Lareu, M. V. ; Barral, S. ; Salas, A. ; Pestoni, C. ; Carracedo, A.
    Springer
    Published 1998
    Staff View
    ISSN:
    1437-1596
    Keywords:
    Key words D1S1656 ; Short tandem repeats ; Human genome ; Sequence variation
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Law
    Notes:
    Abstract A short tandem repeat at the D1S1656 locus was sequenced in 45 selected alleles and 13 different alleles were found which were designated according to the total number of repeats. This STR is a compound hypervariable STR consisting of blocks of (TAGR) repeats with a basic sequence structure (TAGA)4(TGA)0-1(TAGA)6-16- (TAGG)0-1(TG)5. The presence of a TGA, probably due to an A deletion in the fifth TAGA repeat leads to intermediate a.3 alleles. Population data showed that this is a highly polymorphic STR with a heterozygosity of more than 0.89. This fact together with its simple structure and small size (129–168 bp) makes this STR one of the most interesting DNA polymorphisms for forensic and genetic purposes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s004140050161

    Articles: DFG German National Licenses
  18. 18
    Staff View
    ISSN:
    1432-0428
    Keywords:
    Keywords ICAM-1 ; P-selectin ; free radicals ; inflammation ; leucocyte ; endothelium.
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract Aims/hypothesis. Diabetes is associated with a high incidence of ischaemic disease and impaired nitric oxide responses. Therefore, the aim of the present study was to assess the effect of nitric oxide on ischaemia/reperfusion (I/R)-induced microvascular responses in an experimental model of diabetes. Methods. Leucocyte-endothelial cell interactions were studied in mesenteric venules after superior mesenteric artery occlusion (10 min), at 10 and 30 min of reperfusion in control and streptozotocin-induced diabetic rats. An oxidant-sensitive fluorochrome was used to measure oxidant production during reperfusion. P-selectin and ICAM-1 expression were quantified at 10 and 30 min of reperfusion respectively, using radiolabelled monoclonal antibodies. The transcription of ICAM-1 mRNA was determined by northern blot. The effect of spermine NONOate, given locally, on all variables studied, was assessed in additional experiments. Results. Ischaemia/reperfusion induced an enhanced leucocyte accumulation and oxidant production in diabetic animals. Moreover, I/R enhanced endothelial P-selectin expression in both groups of animals, whereas it only up regulated ICAM-1 endothelial expression and mRNA expression in diabetic rats. Spermine NONOate abrogated to a similar extent leucocyte adhesion and emigration in control and diabetic animals, although the mechanisms underlying this protective effect appear to be different. In control rats Spermine NONOate effectively prevented P-selectin up regulation, whereas in diabetic rats NO appreciably attenuated the rapid up regulation of ICAM-1 by preventing its transcription. Conclusions/interpretation. Expression of ICAM-1 is rapidly increased in diabetic, but not control, animals exposed to I/R. The increased endothelial cell adhesion molecule expression, leucocyte-endothelial cell adhesion and oxidant stress induced by I/R in diabetic rats are significantly attenuated by exogenous NO. [Diabetologia (1999) 42: 1350–1358]
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001250051449

    Articles: DFG German National Licenses
  19. 19
    Staff View
    ISSN:
    1432-2161
    Keywords:
    Key words Parosteal osteoma ; Iliac bone ; Computed tomography ; Magnetic resonance imaging ; Partial regression
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract  A 33-year-old patient with a 2-year history of intermittent pain in the right gluteal region and thigh presented with a large sclerotic lesion of the iliac bone. From the findings on radiography, scintigraphy, CT and MRI, a giant parosteal osteoma was suspected. The histological examination confirmed the diagnosis. Since the lesion was extensive it was observed with periodic follow-up examinations. At present, 5 years after the diagnosis, the patient is asymptomatic and imaging studies show that the lesion persists with reduction of sclerosis and size. The tumor was on the surface as well as intramedullary – only one other case with such a distribution is known to us – and it was also in the iliac bone.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002560050357

    Articles: DFG German National Licenses
  20. 20
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words RAPD markers ; Genetic diversity ; Potato gene banks ; Solanum jamesii ; S. fendleri ; APIC ; Wild potato populations ; Ex situ conservation
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  An important question in the conservation of potato germ plasm is whether germ plasm in the gene bank, although stable, still represents the in situ populations from which it was collected, sometimes many decades ago. The answer would direct objective decisions regarding the value of re-collections and in situ preservation. The present study was undertaken as a project of the Association of Potato Inter-gene-bank Collaborators (APIC). It measured genetic differentiation between potato germ plasm maintained in the US gene bank for many years and current in situ populations re-collected from the same original sites in the wild. Solanum jamesii and Solanum  fendleri from the United States were used as representatives of potato germ plasm. Re-collections were carried out in 1992 at the same locations at which gene bank-conserved accessions had been collected in 1958 and 1978. RAPD markers revealed significant genetic differences between gene bank-conserved and re-collected in situ populations for all seven comparisons of S. jamesii (diploid outcrosser), and 12 of 16 comparisons within S.  fendleri (tetraploid inbreeder). The average genetic similarities were 65.2% for S.  jamesii and 80.4% for S.  fendleri. Possible explanations and consequences of these unexpectedly large differences are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050548

    Articles: DFG German National Licenses