Search Results - (Author, Cooperation:A. Bonetti)

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  1. 1
    A. R. Forrest ; H. Kawaji ; M. Rehli ; J. K. Baillie ; M. J. de Hoon ; V. Haberle ; T. Lassmann ; I. V. Kulakovskiy ; M. Lizio ; M. Itoh ; R. Andersson ; C. J. Mungall ; T. F. Meehan ; S. Schmeier ; N. Bertin ; M. Jorgensen ; E. Dimont ; E. Arner ; C. Schmidl ; U. Schaefer ; Y. A. Medvedeva ; C. Plessy ; M. Vitezic ; J. Severin ; C. Semple ; Y. Ishizu ; R. S. Young ; M. Francescatto ; I. Alam ; D. Albanese ; G. M. Altschuler ; T. Arakawa ; J. A. Archer ; P. Arner ; M. Babina ; S. Rennie ; P. J. Balwierz ; A. G. Beckhouse ; S. Pradhan-Bhatt ; J. A. Blake ; A. Blumenthal ; B. Bodega ; A. Bonetti ; J. Briggs ; F. Brombacher ; A. M. Burroughs ; A. Califano ; C. V. Cannistraci ; D. Carbajo ; Y. Chen ; M. Chierici ; Y. Ciani ; H. C. Clevers ; E. Dalla ; C. A. Davis ; M. Detmar ; A. D. Diehl ; T. Dohi ; F. Drablos ; A. S. Edge ; M. Edinger ; K. Ekwall ; M. Endoh ; H. Enomoto ; M. Fagiolini ; L. Fairbairn ; H. Fang ; M. C. Farach-Carson ; G. J. Faulkner ; A. V. Favorov ; M. E. Fisher ; M. C. Frith ; R. Fujita ; S. Fukuda ; C. Furlanello ; M. Furino ; J. Furusawa ; T. B. Geijtenbeek ; A. P. Gibson ; T. Gingeras ; D. Goldowitz ; J. Gough ; S. Guhl ; R. Guler ; S. Gustincich ; T. J. Ha ; M. Hamaguchi ; M. Hara ; M. Harbers ; J. Harshbarger ; A. Hasegawa ; Y. Hasegawa ; T. Hashimoto ; M. Herlyn ; K. J. Hitchens ; S. J. Ho Sui ; O. M. Hofmann ; I. Hoof ; F. Hori ; L. Huminiecki ; K. Iida ; T. Ikawa ; B. R. Jankovic ; H. Jia ; A. Joshi ; G. Jurman ; B. Kaczkowski ; C. Kai ; K. Kaida ; A. Kaiho ; K. Kajiyama ; M. Kanamori-Katayama ; A. S. Kasianov ; T. Kasukawa ; S. Katayama ; S. Kato ; S. Kawaguchi ; H. Kawamoto ; Y. I. Kawamura ; T. Kawashima ; J. S. Kempfle ; T. J. Kenna ; J. Kere ; L. M. Khachigian ; T. Kitamura ; S. P. Klinken ; A. J. Knox ; M. Kojima ; S. Kojima ; N. Kondo ; H. Koseki ; S. Koyasu ; S. Krampitz ; A. Kubosaki ; A. T. Kwon ; J. F. Laros ; W. Lee ; A. Lennartsson ; K. Li ; B. Lilje ; L. Lipovich ; A. Mackay-Sim ; R. Manabe ; J. C. Mar ; B. Marchand ; A. Mathelier ; N. Mejhert ; A. Meynert ; Y. Mizuno ; D. A. de Lima Morais ; H. Morikawa ; M. Morimoto ; K. Moro ; E. Motakis ; H. Motohashi ; C. L. Mummery ; M. Murata ; S. Nagao-Sato ; Y. Nakachi ; F. Nakahara ; T. Nakamura ; Y. Nakamura ; K. Nakazato ; E. van Nimwegen ; N. Ninomiya ; H. Nishiyori ; S. Noma ; T. Noazaki ; S. Ogishima ; N. Ohkura ; H. Ohimiya ; H. Ohno ; M. Ohshima ; M. Okada-Hatakeyama ; Y. Okazaki ; V. Orlando ; D. A. Ovchinnikov ; A. Pain ; R. Passier ; M. Patrikakis ; H. Persson ; S. Piazza ; J. G. Prendergast ; O. J. Rackham ; J. A. Ramilowski ; M. Rashid ; T. Ravasi ; P. Rizzu ; M. Roncador ; S. Roy ; M. B. Rye ; E. Saijyo ; A. Sajantila ; A. Saka ; S. Sakaguchi ; M. Sakai ; H. Sato ; S. Savvi ; A. Saxena ; C. Schneider ; E. A. Schultes ; G. G. Schulze-Tanzil ; A. Schwegmann ; T. Sengstag ; G. Sheng ; H. Shimoji ; Y. Shimoni ; J. W. Shin ; C. Simon ; D. Sugiyama ; T. Sugiyama ; M. Suzuki ; N. Suzuki ; R. K. Swoboda ; P. A. t Hoen ; M. Tagami ; N. Takahashi ; J. Takai ; H. Tanaka ; H. Tatsukawa ; Z. Tatum ; M. Thompson ; H. Toyodo ; T. Toyoda ; E. Valen ; M. van de Wetering ; L. M. van den Berg ; R. Verado ; D. Vijayan ; I. E. Vorontsov ; W. W. Wasserman ; S. Watanabe ; C. A. Wells ; L. N. Winteringham ; E. Wolvetang ; E. J. Wood ; Y. Yamaguchi ; M. Yamamoto ; M. Yoneda ; Y. Yonekura ; S. Yoshida ; S. E. Zabierowski ; P. G. Zhang ; X. Zhao ; S. Zucchelli ; K. M. Summers ; H. Suzuki ; C. O. Daub ; J. Kawai ; P. Heutink ; W. Hide ; T. C. Freeman ; B. Lenhard ; V. B. Bajic ; M. S. Taylor ; V. J. Makeev ; A. Sandelin ; D. A. Hume ; P. Carninci ; Y. Hayashizaki
    Nature Publishing Group (NPG)
    Published 2014
    Staff View
    Publication Date:
    2014-03-29
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; *Atlases as Topic ; Cell Line ; Cells, Cultured ; Cluster Analysis ; Conserved Sequence/genetics ; Gene Expression Regulation/genetics ; Gene Regulatory Networks/genetics ; Genes, Essential/genetics ; Genome/genetics ; Humans ; Mice ; *Molecular Sequence Annotation ; Open Reading Frames/genetics ; Organ Specificity ; Promoter Regions, Genetic/*genetics ; RNA, Messenger/analysis/genetics ; Transcription Factors/metabolism ; Transcription Initiation Site ; Transcription, Genetic/genetics ; Transcriptome/*genetics
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Toffano, G. ; Benvegnù, D. ; Bonetti, A. C. ; Facci, L. ; Leon, A. ; Orlando, P. ; Ghidoni, R. ; Tettamanti, G.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1980
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    The binding of GM1, ganglioside to crude preparations of rat brain neuronal membranes was studied, the following results being obtained: (a) the binding process followed a biphasic kinetics, which displayed a break at 0.07–0.08 X 10−6m GM1, concentration; (b) the features of the binding process at GM1, concentrations below the break and, over the break, above 10-6m appeared to be different. Below the break the process proceeded slowly and brought a stable and irreversible association of GM1, molecules to the membranes. Over 10-6m the process was much more rapid and caused GM1, molecules to interact in such a way that they were releasable by washing and could exchange with newly added free ganglioside; (c) the two binding processes displayed the characteristics of a saturation phenomenon; (d) in both cases, GM1, taken up was freely available to galactose oxidase, indicating that the oligosaccharide chains protrude from the membrane surface. We postulate that GM1, occurs, below and above the break, in different physical forms, each of them having a different mechanism of interaction with the membrane. Above 10-6m GM1, interacts as micelles, and the basis of the micelle-membrane inter action is a fusion process. Below the break, in the 10−8–10−7m range, the binding is the result of hydrophobic interactions between sites on the membrane and the hydrophobic portion of individual ganglioside molecules, most likely in the monomeric form. Toffano G. et al. Interactions of GM1, ganglioside with crude rat brain neuronal membranes. J. Neurochem.35, 861–866 (1980).
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  3. 3
    BELLANDO, M. ; TROTTA, A. ; BONETTI, A. ; COLOMBO, R. ; LADO, P. ; MARRE, E.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1979
    Staff View
    ISSN:
    1365-3040
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Abstract Dissociation of active H+ extrusion (−ΔH+) from K+ uptake in pea and maize root segments was attempted by substituting K+ in the incubation medium with lipophilic cations assumed to enter the cell by passive, non-specific, permeation through the lipid component of the plasmalemma. Among the compounds tested, tributylbenzylammonium significantly stimulated −ΔH+ in the absence of other monovalent cations in the medium. This effect was much more evident when the experiment was carried out in the presence of fusicoccin, which strongly stimulates proton extrusion and monovalent cation uptake, and hyperpolarizes the trans-membrane electric potential in these materials. Also the lipophilic cations tetraphenylphosphonium, dimethyldibenzylammonium and hexylguanidine markedly stimulated FC-promoted −ΔH+. Octylguanidine at a low concentration induced an early stimulation followed by a strong inhibition of −ΔH+. A complete lack of additivity was observed between the effects of lipophilic cations and that of K+ on H+ extrusion. Lipophilic cations severely inhibited K+ uptake.These data are interpreted as supporting the view of an electric, rather than a chemical, (namely, involving the same carrier system) nature of the coupling of active H+ extrusion with K+ influx.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
    COLOMBO, R. ; BONETTI, A. ; LADO, P.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1979
    Staff View
    ISSN:
    1365-3040
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Abstract. The effect of fusicoccin (FC) on the K+stimulated Na+ efflux in root cells of Na+ loaded barley roots was studied. FC (0.02 mM) stimulated Na+ efflux in the presence of K+ and its effect was synergistic with that of K+, in a similar way as its effect on proton extrusion. Decreasing the pH of the elution medium promoted Na+ efflux and partially replaced the effect of FC. As FC is known to increase the electrochemical proton gradient at the plasmalemma level, these results are consistent with the hypothesis that Na+ is extruded in exchange for H+. A further support to this view came from the finding that Na+ efflux was also promoted by a lipophilic cation, tributylbenzylammonium (TBBA +), which stimulates H + extrusion and is generally accepted not to enter the cells by means of the same carrier as K +.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  5. 5
    BENVENUTI, S ; DINELLI, G ; BONETTI, A ; CATIZONE, P

    Oxford, UK : Blackwell Science Ltd
    Published 2005
    Staff View
    ISSN:
    1365-3180
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Trials were carried out to study the germination and dormancy of Cuscuta campestris Y. (dodder) seeds and factors influencing the success of early parasitisation of sugarbeet. Primary dormancy can be removed by seed scarification. Germination was negligible at 10°C and optimal at 30°C, while it was not influenced by light. Seed burial induced a cycle of induction and breaking of secondary dormancy. Seedling emergence was inversely proportional to the depth of seed burial and only seed buried within 5 cm of the soil surface emerged. Storage of C. campestris seeds in a laboratory for 12 years resulted in the loss of primary dormancy, enabling the germination of all viable seeds. Host infection (i.e. protrusion of parasite haustoria from host tissue) was heavily influenced by host growth stage. Tropism towards a host was due to the perception of light transmitted by green parts of sugarbeet plants. Insertion of a transparent glass sheet between host leaves and parasite seedlings did not modify this response. This phototropism permitted Cuscuta to identify host plants with high chlorophyll content as a function of the lower red/far red ratio of transmitted light.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  6. 6
    Benvenuti, S ; Dinelli, G ; Bonetti, A

    Oxford, UK : Blackwell Science Ltd
    Published 2004
    Staff View
    ISSN:
    1365-3180
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Leptochloa chinensis is a new weed that has been found with increasing frequency in Italian rice paddies. The germination ecology of L. chinensis seeds was studied in order to investigate the development mechanisms and survival strategy of this weed in rice paddies of northern Italy. Leptochloa chinensis seeds showed no dormancy and exhibited germination even in anoxic conditions. Germination was strongly influenced by temperature (minimum around 15°C; optimal 25–35°C) and light (phytochrome dependent). Temperature fluctuation caused an increase of seed germination in the dark. Seed burial also strongly inhibited germination and emergence of this species. At 5 cm seed burial only 5% of seedlings emerged in flooded conditions, while at the same depth, but with no flooding, no seedling emergence was observed. This phenomenon was not due to oxygen depletion, as germination was not inhibited by complete anoxia, as demonstrated by the fact that some seedlings did emerge in flooding conditions when water was no deeper than 6 cm. Seed burial and concomitant flooding induced an unusual germination: first coleoptile emergence and subsequently emergence of the radicle was observed. The possible exploitation of this knowledge for weed management is discussed.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  7. 7
    DINELLI, G. ; BONETTI, A. ; TIBILETTI, E.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1993
    Staff View
    ISSN:
    1365-3180
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Photosynthetic and accessory pigments (chlorophyll a and b, a and ß carotenes and xanthophylls) were evaluated in Cuscuta campestris Yuncker during the course of its biological cycle, taking into account both autotrophic and heterotrophic phases, and different parts of the parasite. Chloroplast activity in C. campestris was measured and comparisons were made with the chloroplast activity in Beta vulgaris L. and Convolvulus arvensis L. The effects of parasitization of C. campestris on the main and accessory photosynthetic pigments of B. vulgaris were also evaluated.Finally, a hypothesis was formulated to explain the different distribution of pigments in different parts and at different stages of the growth cycle in C. campestris and its chloroplast activity. Pigments photosynthétiques principaux et accessoires chez Cuscuta campestris Yuncker et des espèces hôtes Les pigments photosynthétiques principaux et accessoires (chlorophylles a et b, α et β carotènes, xanthophylles) ont été déterminés chez Cuscuta campestris Yuncker durant son cycle biologique. Les phases auttorophes et hétérotrophes ont été prises en compte et les mesures ont porté sur différentes parties des plantes. L'activité chloroplastique a été déterminée chez C. campestris et a été comparéà celles de Beta vulgaris L. et de Convolvulus arvensis L. Les conséquences du parasitisme de C. campestris sur les pigments principaux et accessoires de B. vulgaris ont aussi étéétudiées.Enfin, une hypothèse a été avancée pour expliquer les différences de distribution des pigments dans les différentes parties de C. campestrisà divers stades de son cycle biologique, ainsi que son activité chloroplastique. Photosynthese- und akzessorische Pigmentre in Cuscuta campestris Yuncker und einigen WirstspflanzenartenBei Cuscuta campestris Yuncker wurden im Verlaufe ihres Lebenszyklus die Photosynthese- und akzessorischen Pigmenten (Chlorophyll a und b, a- und ß-Karotin, Xanthophylle) sowohl in autotrophen als auch heterotrophen Phasen und in verschiedenen Pflanzenteilen untersucht. Die Chloroplastenaktivität wurde gemessen und mit der von Beta vulgaris L. und Convolvulus arvensis L. vergleichen. Die Wirkung der Parasitierung von Cuscuta campestris auf die Photosynthese-Pigmente von Beta vulgaris wurd erfaßt. Eine Hypothese über die Chloroplastenaktivität und zur verschiedenen Verteilung der Pigmente in den verschiedenen Pflanzenteilen und in verschiedenen Entwicklungsstadien von Cuscuta campestris wurde formuliert.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  8. 8
    Dinelli, G ; Bonetti, A ; Lucchese, C ; Catizone, P ; Bravin, F ; Zanin, G

    Oxford UK : Blackwell Science Ltd
    Published 2002
    Staff View
    ISSN:
    1365-3180
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    A comprehensive research project was set up to characterize by different approaches some Italian populations of Lolium spp. susceptible and resistant to diclofop-methyl. The present study was aimed at the taxonomic identification of these Lolium populations on the basis of the electrophoretic analysis of seed reserve proteins. Electrophoresis confirmed the great range of variation detected by preliminary morphological analysis. Approximately half the individuals in each population could be classified as belonging to Lolium multiflorum or Lolium rigidum, the most common Lolium species present in the area. However, electrophoretic patterns revealed a significant number (40–60%) of hybrid individuals in all populations. The origin of these off-types was postulated as resulting from both intrageneric hybridization among different Lolium species and intergeneric hybridization between Lolium and Festuca genera. No significant differences were detected in the taxonomy of herbicide-susceptible and -resistant populations.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  9. 9
    Bonetti, A.

    Milano : Periodicals Archive Online (PAO)
    Published 1963
    Staff View
    ISSN:
    0001-9593
    Topics:
    Linguistics and Literary Studies
    History
    Notes:
    Recensioni
    URL:
    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    0022-2852
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  11. 11
    Dinelli, G. ; Bonetti, A. ; Catizone, P. ; Galletti, G.C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0378-4347
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  12. 12
    Staff View
    ISSN:
    0273-1177
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  13. 13
    Colombo, R. ; Bonetti, A. ; Cerana, R. ; Lado, P.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0304-4211
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  14. 14
    Cerana, R. ; Bonetti, A. ; Lado, P.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0304-4211
    Keywords:
    Ca^2^+ ; Calmodulin antagonist ; Chlorpromazine membrane binding ; Maize roots
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  15. 15
  16. 16
    Cerana, R. ; Bonetti, A. ; Marrè, M. T. ; Romani, G. ; Lado, P. ; Marrè, E.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1983
    Staff View
    ISSN:
    1399-3054
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Brassinolide, a plant hormone newly isolated from pollen, promotes growth of the stem of a number of plant species. Similar effects are induced by a brassinosteroid (BR), the synthetic 24-epibrassinolide. In this paper the effects of BR on acid secretion and transmembrane electrical potential difference in Azuki bean (Vigna angularis, Ohwi and Ohashi cv. Takara) epicotyls were determined in short term experiments and compared with the effects on growth. At concentrations between 10-7 to 10-5M, BR stimulates, similarly to indole-3-acetic acid (IAA), growth and H+ extrusion and hyperpolarizes the transmembrane electric potential (PD). These effects of BR, as well as those of IAA, are suppressed by inhibitors of RNA and protein synthesis. All these effects of BR and IAA appear roughly additive, even when both hormones are present at their optimal concentrations. The data are interpreted as showing that the action of BR on growth is at least in part mediated by its capability to activate electrogenic proton extrusion. The additivity of the effects of BR and IAA suggests that the primary mechanism of action of the two hormones is different.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  17. 17
    Romani, G. ; Marriè, M. T. ; Bonetti, A. ; Cerana, R. ; Lado, P. ; Marrè, E.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1983
    Staff View
    ISSN:
    1399-3054
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    In apical or subapical root segments of maize (Zea mays L. cv. Dekalb XL640), 10−7–10−5M 24-epibrassinolide (BR), a physiologically active synthetic epimer of the pollen hormone brassinolide, induces a significant stimulation of root growth, associated with an increase of acid secretion. The increase in acid secretion is enhanced by the presence of K+ in the medium and is accompanied by an early, significant hyperpolarization of the transmembrane electric potential (PD), which is completely suppressed by the addition of the protonophore uncoupler FCCP. Similar effects of BR have earlier been reported for shoots, and also for IAA in shoots. Contrariwise, 10−8–10−7M IAA inhibits acid secretion and depolarizes the PD in the maize root segments. This suggests different pathways for the action of the two different hormones on the proton pump.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  18. 18
    Staff View
    ISSN:
    1569-8041
    Keywords:
    cisplatin ; resistance ; ovarian carcinoma
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract Background: Alteration in apoptosis pathways (in particular mutations of p53 gene) may result in resistance of ovarian carcinoma to cisplatin. However, cisplatin resistance is likely to be multifactorial. An understanding of the molecular alterations associated with the development of resistance may be of considerable relevance in an attempt to optimize the therapeutic approach. Study design: Two cisplatin-resistant sublines (IGROV-1/Pt0.5 and IGROV-1/Pt1), both characterized by mutant p53 (Cancer Res 1996; 56: 556–62), but with different degree of resistance were studied in terms of pattern of cross-resistance, susceptibility to drug-induced apoptosis, expression of gluthathione-dependent system, cellular pharmacokinetics, drug-induced DNA damage. The resistance index (ratio between the IC50 of resistant and sensitive cells) after a 96-hour drug exposure was 10 for IGROV-1/Pt0.5 and 14 for IGROV-1/Pt1 cells. Results: Resistant cells were cross-resistant to DNA-damaging agents and, interestingly, they had a collateral sensitivity to Taxol. The cellular response to Taxol paralleled the drug ability to induce apoptosis. The intracellular glutathione level was significantly increased in IGROV-1/Pt cells compared to the sensitive counterpart. In contrast, glutathione S-transferase level was consistently reduced in both sublines. γ-Glutamyl transpeptidase activity, which was lower in resistant than in sensitive cells, was not directly correlated with glutathione level, thus suggesting a complex regulation of cellular glutathione content. In the resistant cells with the highest glutathione content, a reduced level of cisplatin-induced cross-link was found. Analysis of DNA platination revealed a slight decrease of DNA-bound platinum only in IGROV-1/Pt1 cells. Again, this reduction is consistent with a protective role for glutathione. The expression of metallothionein IIa was increased in both resistant variants. Conclusions: Multiple changes are involved in acquired resistance of ovarian carcinoma cells including reduced susceptibility to apoptosis as consequence of inactivation of p53 and expression of defence mechanisms. The relative contribution is related to the degree of drug resistance. In particular, the glutathione-dependent system could have a role only in the development of a high degree of resistance. Finally, the finding that Taxol was very effective in inducing apoptosis in resistant sublines with p53 mutation supports the expression of an intact p53-independent pathway of apoptosis and suggests the pharmacological interest of Taxol in the treatment of p53-mutated tumors.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  19. 19
    Cerana, R. ; Spelta, M. ; Bonetti, A. ; Lado, P.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0168-9452
    Keywords:
    H^+ extrusion ; brassinosteroid ; cell enlargement ; cholesterol ; maize roots
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  20. 20
    Staff View
    ISSN:
    0020-0891
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses