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Leibniz Institute for Science and Mathematics Education, Kiel

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  • 1
    ISSN: 1432-2285
    Keywords: Taxodium distichum ; Immunoblotting ; Immunogold labeling ; Ultrastructure of wood ray cells ; Protein storing vacuoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ultrastructural investigations showed the occurrence of electron-dense intravacuolar aggregations in wood ray cells of Taxodium distichum (L.) L. C. Rich, during the dormant season. Such aggregations were missing in summer. By SDS-PAGE of the crude protein extract of Taxodium, a prominent protein of about 35 kDa was detected before budbreak. This protein disappeared at the time when the new shoots had fully grown out. An antibody was raised against this “storage protein”. In the immunoblot the antibody reacted specifically with the 35 kDa protein. At the electron-microscope level, by immunogold labeling, the electron-dense intravacuolar aggregations were identified as the specific storage sites for the 35 kDa protein. Thus, these vacuoles proved to be “protein storing vacuoles”.
    Type of Medium: Electronic Resource
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