Biochemistry and Biotechnology
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
Pea protoplast dielectrophoretic coefficients were measured in AC fields of different frequencies applied between concentric cylindrical metal electrodes, outer and inner radii, 0.24 mm and 1 mm, respectively. The values for 0.5 M mannitol solutions are: (6.0, 1.0, 5.6, 16.0 and 17.8) X 10-24Fm2 at frequencies 0.05, 0.1, 0.6, 1 and 8 MHz, respectively. The specific polarizabilities (dielectrophoretic coefficients per unit volume of the protoplasts) are: (5.0, 0.7, 4.0, 12.0 and 13.0) X 10-10Fm-1, respectively. Below 10 kHz dielectrophoretic effects were not observed. Cell rotation was observed in the frequency range from 1 kHz to 10 kHz. These results may be of use in cell separation and electrofusion in scientific research and technology.
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