EVALUATION OF THREE LATEX AGGLUTINATION TEST KITS FOR RAPID DETECTION OF CAMPYLOBACTER ISOLATES FROM CHICKEN SAMPLES
FANG, SHAO W. ; WANG, CHAO H. ; HUANG, TSUI P. ; SHIH, DANIEL Y.C.
Oxford, UK : Blackwell Publishing Ltd
Published 1995
Oxford, UK : Blackwell Publishing Ltd
Published 1995
| ISSN: |
1745-4581
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Biology
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| Notes: |
Three commercially available latex agglutination test (LAT) kits for the rapid identification of Campylobacter isolates were evaluated against 87 isolates of Campylobacter spp. and 46 strains of non-Campylobacter bacteria (26 species). The performance of three LAT kits, MicroScreen® Campylobacter (Mercia Diagnostics, Shalford, UK), BBL CampyslideTM (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) and MERITECTM-Campy (jcl) (Meridian Diagnostics, Cincinnati, OH, USA), were compared. All sensitivities for the three LAT kits were 100%. Specificities of both MicroScreen® Campylobacter and BBL CampyslideTM were 100%, but specificity of MERITECTM-Campy (jcl) was only 89. 1/. The detection limit ranges (log CFU/ml) of MicroScreen®. Campylobacter, BBL CampyslideTM and MERITECTM-Campy (jcl) against five Campylobacter strains were 7.7–9.1, 7.4–8.0 and 8.5–9.4, respectively. BBL CampyslideTM was more sensitive (10X) than the other two LAT kits (p 〈 0.01). A one-day procedure was proposed to identify the suspicious colonies on selective agar by performing the LAT kit, catalase, oxidase and morphological observation.
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| Type of Medium: |
Electronic Resource
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| URL: |