Molecular characterization of Lactobacillus casei strains

Ferrero, M. ; Cesena, C. ; Morelli, L. ; Scolari, G. ; Vescovo, M.

Oxford, UK : Blackwell Publishing Ltd
Published 1996
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Three different molecular techniques were used to characterize bacterial strains isolated from dairy products and phenotypically classified as Lactobacillus casei. Some strains were taxonomically identified as Lb. casei and Lb. paracasei using 23S rRNA-targeted probes specific for Lb. casei and Lb. paracasei. Chromosomal DNA was analyzed by pulsed-field gel electrophoresis. Lb. paracasei strains had a variable number of SfiI fragments harboring rRNA genes, while Lb. casei strains showed more homogeneous patterns. The chromosome size of Lb. paracasei ATCC 334 was evaluated to be 2.17 Mb.
Type of Medium:
Electronic Resource
URL: