The polarographic behavior of adenine nucleotides in unbuffered 75% dioxane solution - Okazaki, Y.; Kawaki, H. (Elsevier)
The polarogaphic behavior of phosphoric acid and nucleotides in unbuffered aqueous and 75% dioxane solution was investigated. It seems reasonable to assume that the half-wave potentials and wave heights of nucleotides depend largely on their dissociation constants and also on the inductive character of their phosphatic part.From the polarographic behavior, circular dichroism and nuclear magnetic resonance spectra, the different behavior due to interaction of theophylline and of nicotinic acid, or imidazole, with cyclic AMP was found to depend on the conformation change of cyclic AMP due to the formation of pyridinium or imidazolium ion.The polarographic behavior of the enzymatic destruction of ATP by apyrase was investigated, and it was found that simultaneous identification of the enzymatic reaction products is possible in unbuffered 75 % dioxane solution.
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Keywords: W; V; U