The oxygen uptake of Sarotherodon niloticus L. and the oxygen binding properties of its blood and hemolysate (pisces: cichlidae) - Verheyen, E.; Blust, R.; Doumen, C. (Elsevier)
1. The oxygen consumption of Sarotherodon niloticus L. was found to decline below a critical oxygen concentration of about 2 mg O"2/l.2. An important influence of CO"2 on the oxygen affinity of whole blood was observed at all temperatures between 20 and 35^oC for gas mixtures containing 5.6% CO"2.3. Purified hemolysate showed extremely high oxygen affinities (p"5"0=1.08 mmHg at pH 8.2 and 20^oC). Low cooperativity was observed at all temperatures from 20 to 35^oC, and pH values between 6.5 and 8.2.4. The Bohr effect proved to be important at pH values lower than pH 7.5 (Φ = Δ logP"5"0/ΔpH = -0.58 between pH 6.5 and 7.0 at 35^oC).5. The oxygen affinities show high thermal sensitivity without a marked pH influence (ΔH value for overall oxygenation at pH was -71.7kJ/mol).6. The obtained results are interpreted as adaptations to diurnal variations in ambient temperature and oxygen availability.
http://dx.doi.org/10.1016/0300-9629(85)90158-6
Keywords: W; V