The evolution of titer and specificity of aldosterone binding antibodies in hyperimmunized sheep - Haning, R.; McCracken, J.; Cyr, M.S.; Underwood, R.; Williams, G.; Abraham, G. (Elsevier)
Two sheep were immunized with an aldosterone-21-hemisuccinate bovine serum albumin complex. Four sheep were immunized to an aldosterone-18,21-dihemisuccinate bovine serum albumin complex. All sheep produced anti-aldosterone antibodies in high titer. The development of antibody titers and cross reactions with related steroids were studied for up to 264 days. Three of the six animals died during this period. There was a large between animal variation in both cross reaction and titer of the antibodies produced. Cross reactions tended to decrease, whereas titers tended to increase with protracted immunization. No definite relation was seen between maximum titer and minimum cross reaction.
http://dx.doi.org/10.1016/0039-128X(72)90119-5
Keywords: WW-YZ