Studies on the electron transport system of Moniezia expansa (CESTODA) - Cheah, K.S.; Bryant, C. (Elsevier)
1. Succinoxidase and NADH oxidase systems in a particulate fraction from Moniezia expansa were studied by manometric and spectrophotometric methods.2. The use of redox dyes and inhibitors showed that succinate and NADH oxidation were accomplished by mechanisms which differed from those in mammalian tissues.3. Succinate and NADH were apparently oxidized by different pathways, involving one or more flavoproteins and a cytochrome component, with the production of hydrogen peroxide.4. A ''peroxidase'' was detected in a soluble fraction from the worm.5. Spectrophotometric study showed that the cytochrome component was of the b type, provisionally identified as ''cytochrome 557 (Moniezia expamsa)''.6. Only small amounts of cytochrome-a were found; there was some evidence for the presence of cytochrome-c.7. The oxidation of cytochrome 557 by fumarate in the particulate preparation from Moniezia expansa, following reduction by NADH, has been demonstrated.8. Experiments with radioactive fumarate showed that it was converted to succinate more readily in the presence of NADH.9. Additional reactions resulting in the formation of malate, aspartate, lactate and alanine also occurred.10. Metabolic schemes to account for these reactions are presented, together with an hypothesis attempting to describe anaerobic respiration in large intestinal parasites.
http://dx.doi.org/10.1016/0010-406X(66)90560-3
Keywords: W; V