Nucleotide sequence of bovine preprocathepsin B - A study of polymorphism in the protein coding region - Mordier, S.; Bechet, D.; Roux, M.-P.; Obled, A.; Ferrara, M. (Elsevier)
A cDNA encoding bovine procathepsin B was isolated. The deduced amino acid sequence revealed that a stop (TAG) codon, instead of a Trp-257 codon (TGG), generates in bovine a cathepsin B precursor four amino acids shorter than in other species. Because micro-heterogeneities were previously reported in the cathepsin B primary structure, sequence polymorphism in the protein coding region was then investigated by PCR sequencing of genomic fragments and RNase protection assays. Experiments performed with 12-15 animals of three breeds did not reveal any difference with our cDNA sequence. We conclude that sequence polymorphism in bovine cathepsin B is a rare event, and can only result from the expression of different alleles of a unique gene.
http://linkinghub.elsevier.com/retrieve/pii/0167-4781(93)90205-R
Keywords: W; V; WW-YZ; U