The physicochemical properties and the process of pyrogel formation of IgD pyroglobulin - Ohno, T.; Furukawa, H.; Kanoh, T.; Uchino, H. (Elsevier)
The extremely rare IgD pyroglobulin caused opalescence, lost its antigenicity and acquired more negative charge when heated for 30 min at 56^oC. Exposure to 60^oC for 60 min produced a firm gel. The thermoprecipitability was abolished by the treatment with guanidine, urea and sodium dodecyl sulfate, but not by 2-mercaptoethanol or neuraminidase, and required the presence of both heavy and light chains. However, amino acid analysis showed abnormalities only in the heavy chain. We hypothesize that the irreversible heat-induced aggregation of IgD pyroglobulin is the result of a conformational change, triggered by light chain, which increases the molecular hydrophobicity.
http://linkinghub.elsevier.com/retrieve/pii/0009-8981(86)90190-7
Keywords: WW-YZ