Protein synthesis in vitro with Tetrahymena mitochondrial ribosomes - Allen, N.E.; Suyama, Y. (Elsevier)
Phenylalanine incorporation studies in vitro have established that 80-S ribosomes from Tetrahymena mitochondria function in protein synthesis. Rate of incorporation of [^1^4C]phenylalanine in the presence of poly(U) was approx. 126 pmoles/h per mg ribosomal protein. Incorporation was sensitive to chloramphenicol but not to cycloheximide. Mitochondrial ribosomes were found to be functionally distinct from cytoplasmic ribosomes and capable of synthesizing high-molecular-weight proteins in the absence of poly(U).
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