Immunologic studies on intracellular isoenzymes; The mitochondrial and cytoplasmic leucyl-tRNA synthetases - Chiu, A.O.S.; Suyama, Y. (Elsevier)
The antisera directed toward leucyl-tRNA synthetase partially purified from Tetrahymena mitochondria did not exhibit positive immunologic reactions when tested against the cytoplasmic enzyme in the following criteria: 1, inhibition of the charging activity; 2, precipitation by immunodiffusion; 3, binding by affinity chromatography.Escherichia coli enzyme could not bind to the anti-mitochondrial enzyme affinity column.These data provide evidence that these enzymes do not share common subunits or possess close structural similarity.
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