Uptake of polynucleotides by intact mammalian cells. XII. Stimulation of the uptake of ribopolynucleotides by deoxyribonuclease - Schell, P.L.; Muller, W. (Elsevier)
The uptake of synthetic polyribonucleotides by ascites tumor cells is increased when the cells are pretreated with deoxyribonuclease or when the cells are exposed to mixtures of deoxyribonuclease and polyribonucleotides (W. Muller, Thesis, T.U. Hannover, 1971, in preparation). In both cases this action is effected by the deoxyribonuclease adsorbed to the cellular membrane. This was demonstrated by the removal of the adsorbed deoxyribonuclease from the intact cells by pronase digestion of this enzyme. The enhancement of uptake has been observed for polymers which either adsorb to the outside of the cells (poly(U)) or penetrate into the cells (poly(A).poly(U)). The viability of the cells is not impaired by deoxyribonuclease treatment. The deoxyribonuclease preparation was free from ribonuclease contamination.
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