Detergent effects on the reaction center-B890 complex of Chromatium vinosum and the mode of bacteriochlorophyll binding as revealed from circular dichroism and nuclear magnetic resonance spectroscopy - Nozawa, T.; Ohta, M.; Hatano, M.; Hayashi, H.; Shimada, K. (Elsevier)
Additional effects of detergents have been examined on absorption and circular dichroism (CD) spectra of a reaction center (RC)-B890 complex of a photosynthetic bacterium, Chromatium vinosum. Anionic detergents in rather higher concentrations destroyed both B890 and RC complexes at the same time, and neutral detergents had a relatively small effect on absorption and CD spectra of the RC-B890 complexes. In contrast, zwitterionic and cationic detergents changed the absorption and CD spectra of the B890 complex at lower concentrations, when α-helical structures of the B890 polypeptides were almost conserved. In this case some amino acid side chains showed increasing molecular motions, as detected by ^1^3C-NMR of the RC-B890 complexes from ^1^3C-enriched cultures. NMR results from a reconstitution experiment which used a ^1^3C-enriched B890-polypeptide and bacteriochlorophyll a with natural abundant carbons implied that histidine residues may be associated with the protein environment about the bacteriochlorophyll molecules.
http://linkinghub.elsevier.com/retrieve/pii/0005-2728(86)90190-8
Keywords: W; V; WW-YZ; U