The effect of 1-deoxymannojirimycin on rat liver α-mannosidases - Bischoff, J.; Kornfeld, R. (Elsevier)
The mannose analogue, 1-deoxymannojirimycin, has been tested for its effect on five α-mannosidase activities present in rat liver and shown to be a specific inhibitor of Golgi α-mannosidase I at low μmolar concentrations. Golgi α-mannosidases I and II were assayed in a highly purified Golgi membrane preparation. Endoplasmic* reticulum α-mannosidase activity was measured in a rough endoplasmic reticulum detergent extract. A purified soluble α-mannosidase activity which we believe is derived from the endoplasmic reticulum during tissue homogenization was also tested. And finally, the lysosomal or acidic α-mannosidase was measured in a postnuclear supernatant fraction obtained from rat liver. The results presented here show that 1-deoxymannojirimycin inhibits only Golgi α-mannosidase I, which is consistent with its effect on oligosaccharide processing in vivo (Fuhrmann et al. Nature 1984 307:755-758).
http://dx.doi.org/10.1016/S0006-291X(84)80371-X
Keywords: W; V; U