Ossification in tissue culture - II. Chemical development of the femur of chick embryo in various liquid media - Ito, Y.; Endo, H.; Enomoto, H.; Wakabayashi, K.; Takamura, K. (Elsevier)
Nine-day chick embryo femora were cultivated by the roller-tube method without plasma. The media consisted of chick embryo extract (CEE), or horse serum (HS), or both, each being diluted with Gey's saline solution (GS). After cultivation, the bones were weighed, and phosphorus and hydroxyproline determined to evaluate the degree of calcification and cartilage matrix formation respectively. The estimates were correlated with histological findings of the bones previously reported. 1. When cultivated in CEE, the bones showed little chemical development despite histological survival and successive elongation.2. When cultivated in HS, the bones presented progressive changes of these estimates with varying concentration of HS. As compared with the estimates of the bones grown in medium CEE:HS:GS (1:2:7), however, maximal values of dry weight and hydroxyproline were low despite significantly promoted elongation. On the other hand, the amount of phosphorus was rather higher than that expected from almost entire loss of periosteum, which is essential for calcification.3. When cultivated in the media of 10 per cent CEE and varying concentration of HS, the bones presented a systematic fluctuation in these estimates. A medium CEE:HS:GS (1:5:4) was shown to be optimal for chemical development of the bone. It was in complete agreement with the morphological and histological findings reported previously.
http://dx.doi.org/10.1016/0014-4827(63)90161-7
Keywords: W; WW-YZ