Synaptic and extra-synaptic distribution of histamine H"1-receptors in rat and guinea pig brains - Mizuguchi, H.; Fukui, H.; Yabumoto, M.; Wada, H. (Elsevier)
Localization of histamine H"1-receptors in subcellular fractions from rat and guinea pig brains was examined in a [^3H]mepyramine binding study. Major [^3H]mepyramine binding sites with increased specific activities ([^3H]mepyramine binding vs. protein amount) were recovered from P2 fractions from both rat and guinea pig brains by differential centrifugation. Further subfractionation of both rat and guinea pig P2 fractions by a discontinuous sucrose density gradient centrifugation showed the highest recovery of [^3H]mepyramine binding with further increased specific activities found in synaptic plasma membrane (SPM) fractions. Minor [^3H]mepyramine binding sites with increased specific activities were detected in both rat and guinea pig P3 fractions. [^3H]Mepyramine binding sites in SPM and P3 fractions showed identical Kd values in each species. These results indicate that histamine H"1-receptors are located not only in synaptic but also in extrasynaptic membranes of both rat and guinea pig brains.
http://dx.doi.org/10.1016/0006-291X(91)91524-G
Keywords: W; V; U