Nucleotide sequence of the promotor and NH"2-terminal signal peptide region of Bacillus subtilis α-amylase gene cloned in pUB110 - Ohmura, K.; Yamazaki, H.; Takeichi, Y.; Nakayama, A.; Otozai, K.; Yamane, K.; Yamasaki, M.; Tamura, G. (Elsevier)
The nucleotide sequence of the promotor and NH"2-terminal signal peptide region of the α-amylase gene derived from the α-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH"2-terminal region of the mature α-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the α-amylase gene of B. amyloliquefaciens.
http://dx.doi.org/10.1016/0006-291X(83)91516-4
Keywords: W; V; U