Inhibition of noncapped mRNA translation by the cap analogue, 7-methylguanosine-5'-phosphate - Seal, S.N.; Schmidt, A.; Tomaszewski, M.; Marcus, A. (Elsevier)
The cap analogue, 7-methylguanosine-5'-phosphate (pm^7G), inhibits the translation of the noncapped STNV (satellite tobacco necrosis virus) RNA and CPMV (cowpea mosaic virus) RNA in the in vitro wheat germ protein synthesizing system. While the translation of some capped mRNAs is inhibited more strongly by the analogue, other capped mRNAs have a level of sensitivity similar to that of the noncapped RNAs. Evidence is presented demonstrating that the effect of the analogue is exerted at a cap binding site even when it is inhibiting noncapped mRNAs. These results therefore indicate that the cap binding site of the translational system is either part of or is closely linked to another mRNA binding component, this component being specific for a site on the mRNA other than the 5' cap. The observations also suggest caution in the use of pm^7G inhibition to indicate the presence of a 5' cap on a particular mRNA.
http://dx.doi.org/10.1016/0006-291X(78)90910-5
Keywords: W; V; U