Inhibition of platelet secretion of ATP by phalloidin - Hashimoto, K.; Kawarabayashi, K.; Hashimoto, K.; Tatsumi, N. (Elsevier)
The involvement of actin in the secretion of ATP by platelets was studied using two stimulants, ADP and A23187, and two actin-mediating reagents, cytochalasin B and phalloidin. The degree of actin polymerization was determined using DNase I.Preincubation of platelets with cytochalasin B suppressed the polymerization of actin and ATP secretion induced by stimulants. In the absence of the stimulant, phalloidin-treated platelets exhibited time-dependent actin polymerization and the maximum level was reached at 5 min. No secretion of ATP was observed.The polymerization was enhanced by phalloidin when the platelets were preincubated for 3 to 5 min with the stimulants, but little ATP was secreted. After a 30-min preincubation, the amount of polymerized actin was lower than that after a 5-min incubation, and no ATP was secreted.
http://dx.doi.org/10.1016/0309-1651(86)90018-4
Keywords: W