Construction of a Bovine Genomic Library of Large Yeast Artificial Chromosome Clones - Libert, F.; Lefort, A.; Okimoto, R.; Womack, J.; Georges, M. (Elsevier)
We have constructed a yeast artificial chromosome (YAC) library derived from hull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8 x 12 positions) and in 2 x 224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones. The proportion of chimeric YACs was estimated from the analysis of a subset of the positive clones: 3 of 9 YACs were found to contain noncontiguous DNA.
http://dx.doi.org/10.1006/geno.1993.1465
Keywords: W; WW-YZ