Analysis of the Developmental and Transcriptional Potentiation Functions of 5'HS2 of the Murine β-Globin Locus Control Region in Transgenic Mice - Enver, T.; Li, Q.; Gale, K.B.; Hu, M.; May, G.E.; Karlinsey, J.E.; Jimenez, G.; Papayannopoulou, T.; Costantini, F. (Elsevier)
We analyze the role of 5'HS2 of the mouse β-globin LCR in the transcriptional and developmental regulation of β-globin gene expression. Previous studies have shown that the human β-globin gene behaves as an adult gene in transgenic mice, being expressed in fetal liver and bone marrow-derived erythroblasts but not in yolk sac-derived embryonic erythroid cells. We show that linkage of mLCR5'HS2 to a human β-globin gene alters this pattern of expression during ontogeny, resulting in expression of the linked β-globin gene at all stages of murine erythroid development. Expression was independent of integration position and correlated with transgene copy number. Our results provide the first test of a phylogenetically homologous LCR in transgenic mice and demonstrate evolutionary conservation of both developmental and transcriptional potentiation functions between mammalian β-globin LCRs.
http://dx.doi.org/10.1006/dbio.1994.1277
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