Interactions between staphylococcal protein A and immunoglobulin domains - Lancet, D.; Isenman, D.; Sjodahl, J.; Sjoquist, J.; Pecht, I. (Elsevier)
The affinity and stoichiometry of interaction between staphylococcal protein A and different domains of immunoglobulins have been studied. Light scattering and tryptophan fluorescence quenching titrations along with direct binding assays were performed. The lack of binding to protein A of pFc' fragment (corresponding to C"H3 domain of IgG) or of Facb derivative of rabbit IgG (which is devoid of the C"H3) suggests that the locus of protein A binding is at the interface between the C"H2 and C"H3 domains. This assignment is also supported by results of the tryptophan fluorescence quenching and C1 binding experiments.
http://dx.doi.org/10.1016/0006-291X(78)91206-8
Keywords: W; V; U