Oxidative titrations of Rhus vernicifera laccase and its specific interaction with hydrogen peroxide - Farver, O.; Goldberg, M.; Lancet, D.; Pecht, I. (Elsevier)
The reaction of oxidized Rhus vernicifera laccase and H"2O"2 leads specifically to the formation of a stable, high affinity complex. It is characterized by an absorption band at 325 nm and is most probably formed with the type 3 site. Oxidative titrations of laccase show a different pathway from the reductive ones. This is also expressed in different Nernst coefficients observed for each half of the redox cycle (2 for reduction, 1 for oxidation). Oxidation of the type 3 site by H"2O"2 proceeds in a bimolecular reaction, whereas type 1 is oxidized in an indirect pathway.
http://dx.doi.org/10.1016/0006-291X(76)90734-8
Keywords: W; V; U