Identification of Three Forms of the γ Subunit of G Proteins Isolated from Bovine Spleen - Morishita, R.; Masuda, K.; Niwa, M.; Kato, K.; Asano, T. (Elsevier)
We reported previously that G proteins from bovine spleen contain at least five forms of the γ subunit (designated S1 through S5), among which S1 and S5 have been identified as novel γ and γ"2 subunits, respectively. In this study, we identified the other three forms of the γ subunit, namely, S2, S3 and S4. Amino acid sequences of proteolytic fragments obtained from S2, S3 and S4 coincided exactly with internal sequences of γ"7, γ"5 and γ"2, respectively. However, sequence analysis of undigested S4 revealed that S4 was an N-terminally truncated form of γ"2 while analysis of the C-terminal fragment by fast atom bombardment mass spectrometry revealed that the C-terminus of S4 seemed to be geranylgeranylated and carboxymethylated as is that of intact γ"2. Thus, the three types of γ subunit obtained from bovine spleen were identified as γ"7, γ"5, and an N-terminally truncated γ"2.
http://dx.doi.org/10.1006/bbrc.1993.1953
Keywords: W; V; U