Induction of Photoresponse by the Hydrolysis of Polyphosphoinositides in the Hermissenda Type B Photoreceptor - Sakakibara, M.; Alkon, D.L.; Kouchi, T.; Inoue, H.; Yoshioka, T. (Elsevier)
Direct evidence that the photoresponse of the Hermissenda type B photoreceptor cell is triggered directly by the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP"2) was obtained. Neomycin and spermine, which inhibit PIP"2 breakdown, suppressed light response, while injection of inositol 1,4,5-trisphosphate (IP"3), guanosine 5'-(3-O-thio)triphosphate (GTPγS), guanosine 5'-(2-O-thio)diphosphate (GDPβS), cAMP, cGMP did not alter the light-induced Na^+ influx underlying the photoresponse. Suppression of the photoresponse was also observed with decrease of total amount of membraneous PIP"2 induced by injection of the phosphoinositides (PI) turnover inhibitors, isobutylmethylxanthine (IBMX), LiCl and R 59022.
http://dx.doi.org/10.1006/bbrc.1994.1927
Keywords: W; V; U