Characterization of a highly glycosylated biosynthetic intermediate of ovalbumin - Kato, Y.; Iwase, H.; Hotta, K. (Elsevier)
Ovalbumin extracted from oviduct slices incubated with [^3^5S]methionine or [2-^3]mannose contained two biosynthetic intermediates, OE and OF (). In the present study, these intermediates are further characterized. The ^3H dpm^3^5S dpm ratio of OF labeled with both [^3^5S]methionine and [^3H]mannose was twice that or greater than that of OE. The tritium-labeled OF migrated more slowly than OE on sodium dodecyl sulfate-gel electrophoresis and had a molecular weight exceeding that of OE by about 1500. These findings, along with the fact that [^3H]OF had sugar chains similar to those of [^3H]OE, suggest that OF may possibly have two sugar chains in one molecule. For confirmation of this, the glycosylation sites of OF were examined. Peptic and tryptic glycopeptides were prepared from [2-^3H]mannose-labeled OF and the other ovalbumin subfractions-OA, OC, OD, and OE-and then analyzed by high-performance liquid chromatography. The peptic glycopeptides prepared from [^3H]OF contained glycopeptides in addition to those derived from the other subfractions although tryptic glycopeptides obtained from [^3H]OF were similar to those from the other subfractions. This shows that the above hypothesis is valid.
http://dx.doi.org/10.1016/0003-9861(86)90607-7
Keywords: W; V; U