Retinal cyclic-GMP phosphodiesterase γ-subunit: Use of mutant synthetic peptides to define function - Oppert, B.; Gonzalez, K.; Hurt, D.; Cunnick, J.; Takemoto, D. (Elsevier)
Previously, we have domain-mapped the 87 amino acid PDEγ inhibitory subunit of the retinal phosphodiesterase (PDE) αβγ"2complex using synthetic peptides (1). The PDEγ subunit has a binding domain for transducin-α (Tα) and for PDEα/β within residues #24-45. An inhibitory region for PDEα/β is within residues #80-87. In order to establish the role of individual amino acids in the function of the PDEγ inhibitory subunit, mutants were synthesized and utilized in PDE inhibition assays. The following mutants exhibited a decreased ability to inhibit PDEα/β: Tyr"8"4-〉Gly; Arg"2"4-〉Gly; and Arg"3"3-〉Pro. Sequence comparisons with cone PDEγ indicate that there is identity within these functional regions.
http://dx.doi.org/10.1016/S0006-291X(05)81418-4
Keywords: W; V; U