Spontaneous and enzymic rearrangement of naringenin chalcone to flavanone - N.M. Mol, J.; P. Robbinst, M.; A. Dixon, R.; Veltkamp, E. (Elsevier)
The isomerisation of 2',4,4',6'-tetrahydroxychalcone by the enzyme chalcone isomerase is difficult to assay accurately in view of the spontaneous cyclisation of this chalcone at the alkaline pH optimum of the enzyme. We report here that self-cyclisation of naringenin chalcone is dramatically reduced at pH-values =〈 6.5 and in the presence of high concentrations of serum albumin (5-10 mg ml ^-^1). We have critically evaluated existing assay procedures of chalcone isomerase, utilizing the effects of a monospecific anti-(chalcone isomerase) serum to distinguish between spontaneous and enzymic cyclisation of chalcone. We conclude that the modifications listed above considerably facilitate the measurement of chalcone isomerase kinetics.
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Keywords: W; V