Simple method to prepare non-charged, amphiphilic agarose derivatives, for instance for hydrophobic interaction chromatography - Hjerten, S.; Yao, K.; Liu, Z.-Q.; Yang, D.; Wu, B.-L. (Elsevier)
γ-Glycidoxypropyltrimethoxysilane is coupled in an aqueous medium to agarose via the trimethoxy groups. The epoxide ring can then be employed for attachment of, for instance, alcohols in solutions of dioxane or acetone and in the presence of boron trifluoride diethyl etherate as a catalyst. In the range pH 4-10 the stability of the linkage between the ligand and agarose is similar to that of ligands coupled to agarose via an ether bond. A method is also described where the epoxide reaction with alcohols is catalyzed by stannic chloride. Both catalyst can degrade cross-linked agarose. However, by using appropriate experimental conditions for the stannic chloride-catalyzed reaction the degradation is negligible, which is of importance when high flow-rates are desired as in high-performance liquid chromatography.
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