Identification and determination of imidazole derivatives in cigarette smoke - Moore-Testa, P.; Saint-Jalm, Y.; Testa, A. (Elsevier)
The identification of imidazole derivatives in cigarette smoke was performed by gas chromatography-mass spectrometry (GC-MS) on a fused-silica capillary column coated with Carbowax 20M. A fraction, enriched in imidazoles, was prepared from the smoke condensate by liquid chromatography on Sephadex LH 20 prior to the GC-MS analysis. Ten alklated imidazoles were identified in cigarette smoke, 4(5)-methylimidazole and imidazole being the most abundant.The determination of these compounds was achieved by high-performance liquid chromatography on LiChrosorb Si 60 after chemical derivatization with 4-chloro-7-nitro-benzo-2-oxa-1,3-diazole (NBD-Cl). This method is very selective since no clean-up procedure is necessary. The levels of 4(5)-methylimidazole and imidazole in cigarette smoke condensate vary between 100 and 500 ppm.
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