Interleukin-1β induces synthesis and secretion of interleukin-6 in human chondrocytes - Bender, S.; Haubeck, H.-D.; Van de Leur, E.; Dufhues, G.; Schiel, X.; Lauwerijns, J.; Greiling, H.; Heinrich, P.C. (Elsevier)
Increased concentrations of interleukin-6 (IL-6) have been found in the synovial fluid of patients with osteoarthritis, rheumatoid arthritis and crystal-related joint deseases. It is therefore of great interest to identify the cells responsible for the production of IL-6, and to investigate whether IL-6 plays a role in the pathogenesisof degenerative or inflammatory joint diseases. Here we show that human interleukin-1β (IL-1β) induces IL-6 synthesis and secretion in differentiated human chondrocytes. In organ cultures resembling closely the in vivo system 10^6 chondrocytes incubated with 100 units of interleukin-1β per ml of medium led to the release of 6 x 10^3 units of IL-6 within 24 h. Chondrocytes cultured in agarose or as monolayers similary incubated with IL-1β produced even higher amounts of IL-6: 70 x 10^3 units per 10^6 cells within 24 h. The induction of IL-6 synthesis by IL-1β was also shown at the mRNA level. IL-6 secreted by stimulated chondrocytes showed heterogeneity upon Western blot analysis.
http://linkinghub.elsevier.com/retrieve/pii/0014-5793(90)81404-C
Keywords: W; V; U