Tetrameric structure of the nonactivated glucocorticoid receptor in cell extracts and intact cells - Rexin, M.; Busch, W.; Segnitz, B.; Gehring, U. (Elsevier)
Mouse lymphoma cells contain a nonactivated glucocorticoid receptor of M"r~330000 which is heteromeric in nature and is unable to bind to DNA. Following affinity labeling of the steroid-binding subunit and subsequent cross-linking with dimethyl suberimidate at various times either in cell extracts or in intact cells, a series of labeled bands was detected in SDS gels. From the molecular masses of completely and partially cross-linked complexes we conclude that the large nonactivated receptor is a tetramer composed of two 90 kDa subunits, one 50 kDa polypeptide and one steroid-binding subunit.
http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)81068-8
Keywords: W; V; U