Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8 - Kulesza, H.; Simpson, G.G.; Waugh, R.; Beggs, J.D.; Brown, J.W.S. (Elsevier)
We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight 〉 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The 〉 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.
http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)81315-Q
Keywords: W; V; U