Cloning and expression of a cDNA for the human prostacyclin receptor - Katsuyama, M.; Sugimoto, Y.; Namba, T.; Irie, A.; Negishi, M.; Narumiya, S.; Ichikawa, A. (Elsevier)
A functional cDNA for the human prostacyclin receptor was isolated from a cDNA library of CMK cells, a human megakaryocytic leukaemia cell line. The cDNA encodes a protein consisting of 386 amino acid residues with seven putative transmembrane domains and a deduced molecular weight of 40,956. [^3H]Iloprost specifically bound to the membrane of CHO cells stably expressing the cDNA with a Kd of 3.3 nM. This binding was displaced by unlabelled prostanoids in the order of iloprost = cicaprost carbacyclin prostaglandin E"1 (PGE"1) 〉 STA"2. PGE"2, PGD"2 and PGF"2"α did not inhibit it. Iloprost in a concentration-dependent manner increased the cAMP level and generated inositol trisphosphate in these cells, indicating that this human receptor can couple to multiple signal transduction pathways.
http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)00355-6
Keywords: W; V; U