Selective decrease in low-M"r HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells - Vartiainen, E.; Palvimo, J.; Mahonen, A.; Linnala-Kankkunen, A.; Maenpaa, P.H. (Elsevier)
We have studied the presence of high-mobility-group (HMG) chromatin proteins in undifferentiated F9 mouse teratocarcinoma cells and F9 cells, which were induced to differentiate by treatment with retinoic acid and dibutyryl-cAMP for 5 days. Acetic acid/urea-polyacrylamide gel electrophoresis and reversed-phase HPLC revealed that the induced F9 cells contained 77 and 62% less HMG I and HMG Y, respectively, than their untreated counterparts. The relative amounts of two other low-M"r HMG proteins HMG 14 and HMG 17 remained essentially unchanged and only a minor decrease was observed in the content of one of the high-M"r HMG proteins, HMG 2. The identity of the low-M"r HMG proteins was verified by amino acid analysis or partial sequencing. These results suggest that HMG I and HMG Y are HMG proteins specific for undifferentiated cells.
http://linkinghub.elsevier.com/retrieve/pii/0014-5793(88)80581-7
Keywords: W; V; U