Porcine SINE-associated microsatellite markers: evidence for new artiodactyl SINEs
| ISSN: |
1432-1777
|
|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Biology
Medicine
|
| Notes: |
Abstract Approximately 24% (170/710) of porcine (dG-dT)n·(dC-dA)n microsatellites isolated in our laboratory are associated with a previously described porcine Short Interdispersed Element (SINE) termed PRE-1 SINE. Another 5.6% (40/710) of the microsatellites were adjacent to two previously unidentified SINE sequences, which we have designated ARE-1P (Artiodactyl Repetitive Element-1 Porcine) and ARE-2P. The ARE repeats were also found in bovine microsatellite and genomic sequences in the GenBank database. Genotypic information was obtained from 68.9% of primers where at least one primer sequence was obtained from the PRE-1 SINE and 66.6% of primer pairs designed from the ARE SINEs. The use of primers derived from SINEs significantly increases the number of primer pairs available for genetic linkage studies in swine.
|
| Type of Medium: |
Electronic Resource
|
| URL: |