Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ
| ISSN: |
1617-4623
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| Keywords: |
Regulation of DNA replication ; Coliphageλ ; DnaA initiator ; Transcriptional activation ; Chaperone proteins
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798297393132732416 |
|---|---|
| autor | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| autorsonst | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| book_url | http://dx.doi.org/10.1007/BF02172404 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM205531008 |
| issn | 1617-4623 |
| journal_name | Molecular genetics and genomics |
| materialart | 1 |
| notes | Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition. |
| package_name | Springer |
| publikationsjahr_anzeige | 1996 |
| publikationsjahr_facette | 1996 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1996 |
| publisher | Springer |
| reference | 252 (1996), S. 580-586 |
| schlagwort | Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins |
| search_space | articles |
| shingle_author_1 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| shingle_author_2 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| shingle_author_3 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| shingle_author_4 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. |
| shingle_catch_all_1 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition. 1617-4623 16174623 Springer |
| shingle_catch_all_2 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition. 1617-4623 16174623 Springer |
| shingle_catch_all_3 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition. 1617-4623 16174623 Springer |
| shingle_catch_all_4 | Węgrzyn, G. Pankiewicz, A. Taylor, K. Węgrzyn, A. Taylor, K. Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Regulation of DNA replication Coliphageλ DnaA initiator Transcriptional activation Chaperone proteins Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition. 1617-4623 16174623 Springer |
| shingle_title_1 | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| shingle_title_2 | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| shingle_title_3 | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| shingle_title_4 | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T10:07:16.390Z |
| titel | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| titel_suche | Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ |
| topic | W |
| uid | nat_lic_papers_NLM205531008 |