Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri
| ISSN: |
1432-2307
|
|---|---|
| Keywords: |
Human papillomavirus 16 differentiation dependence ; Separate biopsies
|
| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Medicine
|
| Notes: |
Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma.
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798295993196740608 |
|---|---|
| autor | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| autorsonst | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| book_url | http://dx.doi.org/10.1007/BF00197550 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM204992095 |
| issn | 1432-2307 |
| journal_name | Virchows Archiv |
| materialart | 1 |
| notes | Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma. |
| package_name | Springer |
| publikationsjahr_anzeige | 1994 |
| publikationsjahr_facette | 1994 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1994 |
| publisher | Springer |
| reference | 425 (1994), S. 473-480 |
| schlagwort | Human papillomavirus 16 differentiation dependence Separate biopsies |
| search_space | articles |
| shingle_author_1 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| shingle_author_2 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| shingle_author_3 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| shingle_author_4 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. |
| shingle_catch_all_1 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri Human papillomavirus 16 differentiation dependence Separate biopsies Human papillomavirus 16 differentiation dependence Separate biopsies Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma. 1432-2307 14322307 Springer |
| shingle_catch_all_2 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri Human papillomavirus 16 differentiation dependence Separate biopsies Human papillomavirus 16 differentiation dependence Separate biopsies Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma. 1432-2307 14322307 Springer |
| shingle_catch_all_3 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri Human papillomavirus 16 differentiation dependence Separate biopsies Human papillomavirus 16 differentiation dependence Separate biopsies Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma. 1432-2307 14322307 Springer |
| shingle_catch_all_4 | Kube, D. Janda, J. Johannsmeyer, K. D. Bergmann, S. Kiessling, U. Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri Human papillomavirus 16 differentiation dependence Separate biopsies Human papillomavirus 16 differentiation dependence Separate biopsies Abstract Human papillomavirus (HPV)16-specific nucleic acid sequences were analysed in separate biopsies taken from a patient with a poorly differentiated squamous cell carcinoma of the uterine cervix. Biopsies were obtained from histopathologically normal epithelium adjacent to the carcinomatous epithelium, the primary carcinoma and a metastatic lymph node. Signals characterizing viral DNA and oncogene transcription were obviously differentiation dependent as shown by in situ hybridization of viral nucleic acids and immunofluorescence of epithelial differentiation specific proteins. In histologically normal parts of the epithelium viral DNA was amplified at the transition from basal to maturing cells, whereas E6/E7 genes were actively transcribed mainly in maturing epithelial cells following the basal cell layer. Some of the cells in the primary carcinoma and in the metastatic lymph node expressed involucrin at increased levels. Signals for viral DNA and HPV16-specific E6/E7 transcripts decreased in intensity during differentiation in an inverse relationship to the observed involucrin increase in those cells. The absence of Ki67 in cells expressing large amounts of involucrin as revealed by immunostaining, support the inverse correlation between differentiation of cancer cells, HPV16 replication and E6/E7 transcription. The changes in cytokine expression may indicate an HPV16 associated disruption of normal cytokine expression pattern in the carcinoma. 1432-2307 14322307 Springer |
| shingle_title_1 | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| shingle_title_2 | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| shingle_title_3 | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| shingle_title_4 | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:45:01.020Z |
| titel | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| titel_suche | Characterization of human papillomavirus type 16 activity in separate biopsies from a carcinoma of the cervix uteri |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM204992095 |