Isolation and determination of the ferric iron chelate of ethylenediamine di(o-hydroxyphenylacetic acid) in plant tissues
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1573-5036
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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| Notes: |
Summary A new technique for the extraction and quantitative determination of the ferric iron chelate of ethylenediamine di(o-hydroxyphenylacetic acid) from aqueous plant extracts is described. The metal chelate is salted out with ammonium sulfate and then is extracted into a small volume of n-amyl alcohol. Spectrophotometric data indicate that the extracted chelate has an absorption maximum corresponding to the iron chelate in aqueous solution and adheres to Beer's law in the range of 0 to 150 micrograms of the metal chelate. The chelate, being anionic in nature, can be absorbed and desorbed from an anion-exchange resin. Isolation and determination of the iron chelate in tomato tissues also is described. Recovery of the iron chelate from these tissues was 94 to 97 per cent.
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| Type of Medium: |
Electronic Resource
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| URL: |